Rabbit Polyclonal (DANRE)map1lc3b(N-term) Antibody

The DANRE (Danio rerio) MAP1LC3B (N-term) antibody is designed to detect the N-terminal region of microtubule-associated protein 1 light chain 3 beta (MAP1LC3B/LC3B), a key marker of autophagosome formation in autophagy. LC3B, a mammalian ortholog of yeast Atg8. exists in cytosolic (LC3B-I) and lipidated, membrane-bound (LC3B-II) forms, with LC3B-II serving as a critical indicator of autophagic activity. In zebrafish (Danio rerio), LC3B homologs play conserved roles in cellular homeostasis, development, and stress responses. This antibody specifically targets the N-terminal epitope of zebrafish LC3B, enabling researchers to monitor autophagic flux in zebrafish models through techniques like Western blotting, immunofluorescence, or immunohistochemistry. Its application is vital in studies investigating autophagy-related mechanisms in zebrafish, such as nutrient deprivation, hypoxia, or disease modeling (e.g., neurodegeneration, cancer). Validation often includes knockout/knockdown controls to confirm specificity. As autophagy research expands in zebrafish due to their genetic tractability and translucency for live imaging, this antibody serves as a crucial tool for visualizing dynamic autophagic processes in vivo. Its N-terminal targeting ensures compatibility with detection methods requiring epitope accessibility, distinguishing it from antibodies binding to LC3B's C-terminal region, which may be obscured during lipid conjugation.