| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A, Htr2a, Htr2 |
| Entrez GeneID | 15558 |
| WB Predicted band size | 52.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Htr2a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human Htr2a. |
+ +
以下是关于Htr2a (N-term)抗体的3篇参考文献及其摘要概括：
---
1. **文献名称**：*"Production of a polyclonal antibody against the N-terminal region of the 5-HT2A receptor and its application in brain tissue analysis"*
**作者**：Smith, J. et al.
**摘要**：研究团队开发了一种针对5-HT2A受体N端(1-50氨基酸)的多克隆抗体，并通过Western blot和免疫组化验证其特异性。结果显示该抗体能特异性识别大鼠前额叶皮层中的5-HT2A受体，未与其他5-HT受体亚型发生交叉反应。
---
2. **文献名称**：*"Characterization of an anti-N-terminal 5-HT2A receptor antibody for studying receptor trafficking in live neurons"*
**作者**：Lee, S. & Kim, H.
**摘要**：本文报道了一种新型单克隆抗体，靶向5-HT2A受体N端表位，用于实时追踪受体在神经元细胞膜上的动态分布。实验表明，该抗体可有效标记内源性受体，并揭示其在激动剂刺激后的内吞途径。
---
3. **文献名称**：*"Differential expression of 5-HT2A receptor isoforms in human prefrontal cortex revealed by N-terminal-specific antibodies"*
**作者**：Garcia, R. et al.
**摘要**：通过使用两种N-term特异性抗体，研究者发现人类前额叶皮层中5-HT2A受体存在不同剪接变体，并证实其表达水平与精神疾病患者脑组织中的蛋白丰度变化相关。抗体的特异性通过siRNA敲低实验进一步验证。
---
**备注**：上述文献为示例，实际引用时需根据具体研究补充真实文献信息。建议通过PubMed或Web of Science以关键词“Htr2a antibody N-terminal”检索最新文章。
The Htr2a (N-term) antibody is a specialized tool designed to target the N-terminal region of the 5-hydroxytryptamine 2A receptor (5-HT2A), a G protein-coupled receptor (GPCR) critical in serotonin-mediated signaling. Expressed predominantly in the central nervous system, the 5-HT2A receptor regulates diverse physiological processes, including mood, cognition, and perception, and is implicated in psychiatric disorders such as schizophrenia, depression, and anxiety. This antibody is generated against a synthetic peptide corresponding to the extracellular N-terminal domain of the receptor, ensuring specificity for distinguishing 5-HT2A from closely related receptors like 5-HT2C.
Widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence, the Htr2a (N-term) antibody facilitates the study of receptor expression, localization, and trafficking in brain tissues, cell lines, or transfected systems. Its application extends to both basic research and drug discovery, particularly in exploring the mechanisms of psychedelics, antipsychotics, and antidepressants that modulate 5-HT2A activity. Validation via knockout controls or siRNA knockdown is recommended to confirm signal specificity. By enabling precise detection of 5-HT2A, this antibody supports advancements in understanding serotonin signaling and its therapeutic targeting.
×
