Rabbit Polyclonal Cry2 Antibody

+ +

•  

   
     Western blot analysis of lysates from human skeletal muscle and human heart tissue (from left to right),  using Cry2 Antibody (R579)(Cat.  #P32958).  P32958 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.    

•  

   
     Western blot analysis of lysates from human heart, skeletal muscle and liver tissue lysate (from left to right),  using Cry2 Antibody(R579)(Cat.  #P32958).  P32958 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.    

•  

   
     Western blot analysis of hCry2-R579 (Cat. #P32958) in  mouse liver tissue and HepG2 cell line lysates (35ug/lane). CRY2 (arrow) was detected using the purified Pab.    

•  

   
     Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.    

**Background of Cry2 Antibodies**

Cry2 antibodies are immunological tools designed to detect and study Cry2 proteins, a class of δ-endotoxins produced by the bacterium *Bacillus thuringiensis* (Bt). These proteins are widely utilized in agriculture as bioinsecticides due to their specificity against insect pests, particularly Lepidoptera and Diptera. Cry2 toxins, such as Cry2Ab and Cry2Ac, form crystalline inclusions during bacterial sporulation. Upon ingestion by target insects, the toxins are solubilized and activated in the alkaline midgut, binding to specific receptors and disrupting epithelial cell integrity, leading to insect death.

Cry2 antibodies are critical for monitoring Cry2 expression in genetically modified (GM) crops (e.g., Bt cotton and maize) and assessing environmental persistence or food safety. They are typically generated by immunizing animals with purified Cry2 proteins or recombinant fragments, yielding polyclonal or monoclonal antibodies with high specificity. These antibodies enable techniques like ELISA, Western blotting, and immunoassays to quantify Cry2 levels in plant tissues, soil, or food products.

Recent research focuses on optimizing antibody sensitivity and cross-reactivity profiles, as Cry2 variants share structural homology. Challenges include ensuring specificity to avoid cross-detection of non-target Cry proteins and addressing insect resistance mechanisms. Advances in recombinant antibody engineering, such as phage display, may enhance Cry2 antibody performance, supporting sustainable pest management and regulatory compliance in agrobiotechnology.