Rabbit Polyclonal (Mouse)Epcam Antibody

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     Western blot analysis of lysates from mouse kidney, mouse lung, mouse testis tissue lysate (from left to right),  using Epcam Antibody (C-term)(Cat.  #P32953).  P32953 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.    

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     Overlay histogram showing HepG2 cells stained with P32953 (red line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P32953,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P32953 staining Epcam in Human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

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     P32953 staining Epcam in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,   paraffin-embedded sections).   Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.  5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).   Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.   A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

EpCAM (epithelial cell adhesion molecule), also known as CD326. is a transmembrane glycoprotein encoded by the *TACSTD1* gene. It plays a dual role in cell adhesion and intracellular signaling, primarily expressed in epithelial tissues during embryonic development and in adult epithelial-derived organs. EpCAM is characterized by its extracellular epidermal growth factor (EGF)- and thyroglobulin-like domains, a single transmembrane region, and a short intracellular domain. It mediates homophilic cell-cell adhesion and regulates processes like proliferation, differentiation, and stemness via cleavage-dependent signaling pathways.

Mouse EpCAM antibodies are critical tools for studying EpCAM biology in murine models, which are widely used in cancer and epithelial research. These antibodies recognize conserved epitopes in mouse EpCAM, enabling applications such as flow cytometry, immunohistochemistry, and Western blotting. EpCAM is overexpressed in many epithelial-derived cancers (e.g., colorectal, breast), making it a biomarker for tumor-initiating cells (cancer stem cells) and a therapeutic target. In mice, EpCAM antibodies like clone G8.8 or Epab1-3 are used to isolate epithelial cells, track tumor metastasis, or assess tissue regeneration.

Research using mouse EpCAM antibodies has revealed its role in maintaining epithelial integrity, modulating Wnt/β-catenin signaling, and promoting tumor aggressiveness. However, EpCAM’s expression varies across tissues and cancer subtypes, requiring context-specific validation. Recent studies also explore its utility in antibody-drug conjugates or bispecific antibodies for cancer immunotherapy.