| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNA polymerase-associated protein LEO1, Leo1, Gm185 |
| Entrez GeneID | 235497 |
| WB Predicted band size | 75.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Leo1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 616-650 amino acids from the C-terminal region of Mouse Leo1. |
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以下是关于小鼠Leo1抗体的3篇参考文献的简要信息(基于模拟数据，非真实文献)：
1. **文献名称**: "Leo1 interacts with the PAF complex to regulate transcriptional elongation in embryonic stem cells"
**作者**: Zhang et al.
**摘要**: 研究利用小鼠Leo1抗体进行ChIP-seq分析，揭示了Leo1作为PAF复合物亚基在胚胎干细胞中调控转录延伸及维持多能性的作用，并证实其通过组蛋白H2B泛素化修饰影响染色质动态。
2. **文献名称**: "PAF complex subunit Leo1 links oncogenic signaling to epigenetic regulation in lung adenocarcinoma"
**作者**: Wang et al.
**摘要**: 通过免疫沉淀(IP)结合小鼠Leo1抗体，发现Leo1在肺癌中异常高表达，且与Kras信号通路协同调控致癌基因的异常转录，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: "Epigenetic regulation by Leo1 governs neural progenitor differentiation"
**作者**: Chen et al.
**摘要**: 研究利用Leo1条件敲除小鼠模型及抗体染色，证明Leo1通过PAF复合物调控神经前体细胞的增殖和分化，影响发育过程中关键神经基因的表达。
4. **文献名称**: "Characterization of a monoclonal antibody against mouse Leo1 for chromatin association studies"
**作者**: Thompson et al.
**摘要**: 本文报道了一种高特异性小鼠Leo1单克隆抗体的开发与验证，证实其在Western blot、免疫荧光及ChIP实验中的有效性，为研究Leo1在染色质重塑中的作用提供了工具。
(注：以上文献为示例性内容，实际引用需查询真实数据库。)
The Mouse Leo1 antibody is a tool used to study the Leo1 protein, a conserved subunit of the RNA polymerase II-associated factor 1 (Paf1) complex in mammals. Leo1 (also known as PAFL) was initially identified in yeast and plays a critical role in transcriptional elongation, histone modification, and chromatin remodeling. In mice and humans, the Paf1 complex, comprising Leo1. Ctr9. Paf1. Cdc73. and Rtf1. regulates gene expression by interacting with RNA polymerase II and facilitating recruitment of histone-modifying enzymes, such as Set1/COMPASS for H3K4 methylation. Leo1 specifically contributes to transcriptional termination, RNA 3'-end processing, and maintenance of epigenetic marks.
Mouse Leo1 antibodies are commonly raised against recombinant or synthetic peptide antigens corresponding to conserved regions of the murine Leo1 protein. These antibodies enable detection and localization of Leo1 in techniques like Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP). They are particularly valuable in studying embryonic development, stem cell differentiation, and cancer biology, as dysregulation of the Paf1 complex is linked to transcriptional abnormalities in these contexts. Validated for specificity in mouse models, these antibodies help dissect Leo1's role in coordinating transcriptional and epigenetic mechanisms.
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