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Rabbit Polyclonal MouseCdk8 Antibody

  • 中文名: Mouse Cdk8抗体
  • 别    名: Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Cdk8
货号: IPDX32955
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Cdk8
Entrez GeneID264064
WB Predicted band size53.2kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis Mouse Cdk8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 424-458 amino acids from the C-terminal region of Mouse Cdk8.

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参考文献

以下是模拟生成的关于Mouse Cdk8抗体的参考文献示例(建议通过学术数据库核实具体文献):

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1. **文献名称**: *CDK8 regulates β-catenin activity in murine intestinal stem cells*

**作者**: Johnson R, et al.

**摘要**: 该研究利用特异性Mouse Cdk8抗体,通过免疫沉淀和Western blot分析,揭示了CDK8通过调控β-catenin信号通路在小鼠肠道干细胞自我更新中的作用。

2. **文献名称**: *Development and validation of a mouse-specific CDK8 antibody for immunofluorescence studies*

**作者**: Tanaka M, et al.

**摘要**: 本文报道了一种高亲和力Mouse Cdk8单克隆抗体的开发,验证了其在冰冻切片和活细胞成像中的应用,证实CDK8在小鼠胚胎发育中的核内动态定位。

3. **文献名称**: *CDK8 modulates chemotherapy response in mouse models of pancreatic cancer*

**作者**: Gupta S, et al.

**摘要**: 通过免疫组化(使用Mouse Cdk8抗体)和基因敲除实验,证明CDK8表达水平与胰腺癌小鼠模型对吉西他滨的敏感性呈负相关。

4. **文献名称**: *CDK8/19 inhibition enhances reprogramming of mouse somatic cells to pluripotency*

**作者**: Wang L, et al.

**摘要**: 研究采用Mouse Cdk8抗体进行蛋白质表达分析,发现抑制CDK8/19可提高小鼠体细胞重编程为多能干细胞的效率,机制涉及中介体复合物功能调控。

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**注意**:以上文献为示例性质,实际引用时请通过 **PubMed/Google Scholar** 等平台以“CDK8 antibody mouse”等关键词检索,并核对抗体货号(如Santa Cruz的sc-1523)或应用场景(如ChIP、IF)的匹配文献。

背景信息

The mouse CDK8 antibody is a crucial tool for studying the role of Cyclin-Dependent Kinase 8 (CDK8) in transcriptional regulation and cellular processes. CDK8. a member of the CDK family, functions as a kinase module component of the Mediator complex, which bridges transcription factors and RNA polymerase II. It modulates gene expression by phosphorylating transcription regulators (e.g., STAT, SMAD) and interacting with signaling pathways like Wnt/β-catenin. Dysregulation of CDK8 is linked to cancers, metabolic diseases, and developmental disorders. Mouse-specific CDK8 antibodies are designed to detect endogenous CDK8 protein in murine models, enabling research into its tissue-specific functions, kinase activity, and interactions. These antibodies are typically validated for applications such as Western blotting, immunoprecipitation, and immunohistochemistry. Epitope specificity (e.g., C-terminal or kinase domain) and cross-reactivity with related CDKs (e.g., CDK19) must be confirmed via knockout controls. Their development supports mechanistic studies in oncology, stem cell biology, and drug discovery, particularly for CDK8-targeted therapies. Commercial variants may differ in clonality (monoclonal/polyclonal), host species (rabbit, rat), and conjugation (e.g., HRP, fluorescent tags), requiring validation for experimental consistency.

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