Mouse Monoclonal CHRM2 Antibody

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     Western blot analysis of lysates from SH-SY5Y cell line, human brain, mouse brain tissue(from left to right), using CHRM2 Antibody(Cat.  #P32873). P32873 was diluted at 1:500 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20μg per lane.    

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     Fluorescent image of SH-SY5Y cells stained with CHRM2 Antibody (Cat#P32873 ). P32873 was diluted at 1:25 dilution.  An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).    

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     Overlay histogram showing SH-SY5Y cells stained with  (green line).  The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was  Alexa Fluor® 488 goat anti-mouse lgG (166821) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The CHRM2 antibody targets the M2 muscarinic acetylcholine receptor, a G protein-coupled receptor (GPCR) predominantly expressed in the heart, smooth muscles, and central nervous system. CHRM2 binds acetylcholine, mediating parasympathetic nervous system responses such as heart rate reduction, smooth muscle contraction, and modulation of cognitive functions. It primarily couples with inhibitory Gi/o proteins, suppressing cAMP production and activating potassium channels. Dysregulation of CHRM2 is linked to cardiovascular diseases, neuropsychiatric disorders (e.g., schizophrenia, depression), and neurodegenerative conditions like Alzheimer’s disease.

CHRM2 antibodies are essential tools for studying receptor expression, localization, and signaling in research. They are used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess protein levels in tissues or cell lines. Specificity is validated through knockout controls or blocking peptides. Additionally, these antibodies aid in exploring CHRM2's role in drug responses, such as anticholinergic therapies, and its interactions with autoantibodies in autoimmune disorders. By enabling precise detection, CHRM2 antibodies contribute to understanding receptor biology and developing targeted therapies for related diseases.