Mouse Monoclonal SOX2 Antibody

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     Western blot analysis of lysate from SOX2 protein, using SOX2 Antibody(Cat.  #P32871). P32871 was diluted at 1:4000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.    

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     Western blot analysis of SOX2 Antibody (Cat.#P32871) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.    

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     Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#P32871). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)    

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     Fluorescent image of A549 cell stained with SOX2 Antibody(Cat#P32871/SG110310AA).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.SOX2 immunoreactivity is localized to Nucleus significantly.    

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     Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.    

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     Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat.#P32871), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.    

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     Immunohistochemical analysis of paraffin-embedded Human Lung squamous cell carcinoma section using Pink1(Cat#am2048a). am2048a was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.    

**Background of SOX2 Antibody**

SOX2 (SRY-box transcription factor 2) is a critical transcription factor encoded by the *SOX2* gene, belonging to the SOX gene family characterized by a high-mobility group (HMG) DNA-binding domain. It plays a pivotal role in maintaining pluripotency and self-renewal in embryonic stem cells and neural progenitor cells, and is essential for early embryogenesis, particularly in neural and ocular development. Dysregulation of SOX2 is linked to developmental disorders, such as anophthalmia/microphthalmia, and cancers, including gliomas, lung carcinomas, and squamous cell carcinomas, where it promotes tumorigenesis and stem cell-like properties.

SOX2 antibodies are essential tools in research and diagnostics, enabling the detection and localization of SOX2 protein in tissues or cells via techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. These antibodies aid in studying SOX2's role in stem cell biology, cancer progression, and regenerative medicine. In clinical pathology, SOX2 immunostaining is used to identify SOX2-expressing tumors, aiding in differential diagnosis and prognosis. Validation of SOX2 antibodies is crucial, as specificity and sensitivity vary across clones, potentially affecting experimental reproducibility. Overall, SOX2 antibodies bridge mechanistic insights into development and disease with translational applications in biomedicine.