| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Enhancer of mRNA-decapping protein 3, LSM16 protein homolog, YjeF domain-containing protein 1, edc3, lsm16, yjdc |
| WB Predicted band size | 54.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) edc3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 162-197 amino acids from the Central region of (DANRE) edc3. |
+ +
以下是关于斑马鱼(Danio rerio)EDC3抗体的示例参考文献(内容为模拟示例，非真实文献)：
---
1. **文献名称**: *EDC3参与斑马鱼胚胎mRNA降解的机制研究*
**作者**: Zhang L, et al.
**摘要**: 本研究利用特异性抗EDC3抗体，通过免疫沉淀和Western blot技术，揭示了斑马鱼早期胚胎中EDC3蛋白在mRNA脱腺苷化和降解过程中的关键作用，并证明其缺失导致胚胎发育异常。
2. **文献名称**: *斑马鱼EDC3抗体的制备及其在组织表达分析中的应用*
**作者**: Wang Y, et al.
**摘要**: 文章报道了一种新型斑马鱼EDC3多克隆抗体的开发，通过免疫组化实验证实EDC3在斑马鱼神经系统和生殖细胞中的高表达，提示其可能在细胞分化中具有调控功能。
3. **文献名称**: *EDC3与斑马鱼母源mRNA清除的关联性研究*
**作者**: Müller S, et al.
**摘要**: 利用EDC3抗体进行共聚焦显微成像，发现EDC3在斑马鱼卵母细胞中与DCP1A和LSM14A共定位，支持其在母源mRNA降解和胚胎发育启动中的必要性。
4. **文献名称**: *CRISPR敲除EDC3对斑马鱼胚胎发育的影响*
**作者**: Chen H, et al.
**摘要**: 结合EDC3抗体验证蛋白敲除效率，研究显示EDC3缺失导致斑马鱼胚胎出现神经管闭合缺陷，并伴随特定mRNA的异常积累，证实其对胚胎早期发育的调控作用。
---
注：以上文献为假设性示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
The DANRE (EDC3) antibody is a research tool developed to study the protein Enhancer of mRNA Decapping 3 (EDC3) in the zebrafish model organism *Danio rerio* (DANRE). EDC3 is a conserved component of the mRNA decapping complex, which plays a critical role in post-transcriptional gene regulation by facilitating the removal of the 5' cap from mRNAs, marking them for degradation. This process is essential for controlling mRNA turnover and fine-tuning gene expression during development and stress responses.
In zebrafish, EDC3 is implicated in embryogenesis, neural development, and germ cell maturation. The DANRE EDC3 antibody is specifically designed to detect endogenous zebrafish EDC3 protein in applications such as Western blotting, immunofluorescence, and immunohistochemistry. Its development often involves immunizing hosts with zebrafish-derived EDC3 epitopes to ensure species-specific recognition.
Researchers use this antibody to explore EDC3's role in mRNA metabolism pathways, its interaction with decapping co-factors (e.g., DCP1A, DDX6), and its involvement in developmental defects or diseases linked to dysregulated mRNA decay. The antibody’s validation typically includes testing in EDC3 knockout models to confirm specificity. By enabling precise localization and quantification of EDC3 in zebrafish tissues, this tool supports studies bridging mRNA dynamics with vertebrate development and disease mechanisms.
×
