| WB | 1/1000 | Human,Mouse,zebrafish, |
| IF | 咨询技术 | Human,Mouse,zebrafish, |
| IHC | 咨询技术 | Human,Mouse,zebrafish, |
| ICC | 技术咨询 | Human,Mouse,zebrafish, |
| FCM | 咨询技术 | Human,Mouse,zebrafish, |
| Elisa | 咨询技术 | Human,Mouse,zebrafish, |
| Aliases | Endothelial differentiation-related factor 1 homolog, EDF-1, edf1 |
| Entrez GeneID | 793036 |
| WB Predicted band size | 16.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,zebrafish, |
| Immunogen | This (DANRE)edf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 122-146 amino acids from the C-terminal region of (DANRE)edf1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(DANRE)EDF1 Antibody(C-term)的参考文献示例(注：由于具体文献的检索限制，以下内容为模拟示例，建议通过学术数据库进一步验证)：
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1. **标题**: *"Endothelial Differentiation Factor 1 (EDF1) regulates vascular development in zebrafish embryos"*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用针对EDF1 C端结构域的多克隆抗体，通过免疫组化和Western blot分析，揭示了EDF1在斑马鱼胚胎血管形成中的关键作用。抗体特异性通过基因敲除实验验证，证明其在体内外功能研究中的可靠性。
2. **标题**: *"Characterization of a novel anti-EDF1 antibody for studying TGF-β signaling in zebrafish"*
**作者**: Smith JL, et al.
**摘要**: 文章报道了一种针对EDF1 C末端的兔源多克隆抗体的开发与验证。该抗体成功应用于斑马鱼胚胎组织的免疫荧光染色，证实EDF1在TGF-β信号通路中与Smad蛋白的相互作用。
3. **标题**: *"EDF1 modulates cardiac development via endothelial-mesenchymal transition in zebrafish"*
**作者**: Chen R, et al.
**摘要**: 研究使用C端特异性EDF1抗体，结合CRISPR/Cas9技术，证明EDF1通过调控内皮-间充质转化(EndMT)影响斑马鱼心脏发育。抗体在蛋白定位和功能缺失实验中表现出高特异性。
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**建议**：如需具体文献，可通过PubMed或Google Scholar以关键词“zebrafish EDF1 antibody C-terminal”检索，或查阅相关抗体供应商(如Sigma-Aldrich、Abcam)提供的文献引用列表。
The DANRE/edf1 Antibody (C-term) is a polyclonal antibody designed to target the C-terminal region of the Edf1 (Ets2-dependent factor 1) protein in *Danio rerio* (zebrafish). Edf1. also known as ERBB receptor feedback inhibitor 1 (ERRFI1), is a conserved regulatory protein involved in modulating receptor tyrosine kinase (RTK) signaling pathways, particularly the EGFR/ERBB family. It acts as a negative feedback regulator by binding to and inhibiting ERBB receptors, thereby influencing cell proliferation, differentiation, and apoptosis. In zebrafish, Edf1 plays critical roles during embryonic development, including tissue patterning and organogenesis, and is implicated in studies of developmental disorders or cancer models.
This antibody is commonly used in applications such as Western blotting, immunohistochemistry, and immunofluorescence to detect endogenous Edf1 expression in zebrafish tissues. Its specificity for the C-terminal region ensures recognition of full-length or truncated protein variants, aiding in studies of post-translational modifications or functional domain analysis. Researchers utilize this tool to explore Edf1's interaction networks, its regulatory effects on signaling cascades, and its potential role in disease mechanisms. By enabling precise localization and quantification of Edf1. the antibody contributes to advancing zebrafish-based models for developmental biology and human disease research.
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