| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Adenylate kinase 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03168}, AK 2 {ECO:0000255|HAMAP-Rule:MF_03168}, 2743 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP-AMP transphosphorylase 2 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP:AMP phosphotransferase {ECO:0000255|HAMAP-Rule:MF_03168}, Adenylate monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03168}, ak2 |
| Entrez GeneID | 321793 |
| WB Predicted band size | 26.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) ak2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of (DANRE) ak2. |
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以下是关于斑马鱼(DANRE)AK2 (N-term)抗体的3篇参考文献的简要概括：
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1. **文献名称**：*Adenylate kinase 2 regulates heart development in zebrafish*
**作者**：Smith J, Doe R, Lee T.
**摘要**：本研究利用针对AK2蛋白N端结构域的特异性抗体，通过免疫印迹和免疫组化技术，揭示了斑马鱼AK2在心脏发育中的关键作用。实验表明，AK2缺失导致心肌细胞能量代谢异常及心脏形态缺陷。
2. **文献名称**：*Characterization of a novel polyclonal antibody targeting the N-terminal domain of zebrafish AK2*
**作者**：Johnson A, Chen X, Wang L.
**摘要**：作者开发并验证了一种针对斑马鱼AK2 N端的多克隆抗体，证实其特异性及在免疫沉淀和免疫荧光中的应用。该抗体被用于研究AK2在斑马鱼胚胎早期能量代谢中的动态表达模式。
3. **文献名称**：*Mitochondrial AK2 activity modulates embryonic development in Danio rerio*
**作者**：García S, Kim M, Patel R.
**摘要**：通过N-term AK2抗体的应用，研究发现斑马鱼AK2在线粒体中的定位对其胚胎发育至关重要。抗体介导的功能抑制实验显示，AK2活性缺失会引发胚胎发育停滞及凋亡信号激活。
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注：上述文献为示例，实际引用时需根据具体研究补充真实发表的论文信息。建议通过PubMed或Web of Science以“zebrafish AK2 antibody”或“Danio rerio adenylate kinase 2”为关键词检索最新文献。
The DANRE (AK2) N-term antibody is designed to target the N-terminal region of adenylate kinase 2 (AK2) in *Danio rerio* (zebrafish), a model organism widely used in developmental biology and disease research. AK2 is a mitochondrial enzyme involved in nucleotide metabolism, catalyzing the reversible transfer of phosphate groups between adenosine phosphates (e.g., ATP and AMP), thus maintaining cellular energy homeostasis. It plays critical roles in processes such as cell proliferation, differentiation, and apoptosis. In zebrafish, AK2 is implicated in early embryonic development, particularly in tissues with high energy demands, including the heart and brain.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study AK2 expression patterns, subcellular localization, and functional roles in zebrafish models. Researchers employ it to explore AK2's involvement in metabolic disorders, mitochondrial dysfunction, and diseases linked to AK2 mutations, such as reticular dysgenesis (a severe immunodeficiency syndrome in humans). The N-terminal specificity ensures recognition of unique epitopes, aiding in distinguishing AK2 from other adenylate kinase isoforms.
Developed using immunogenic peptides or recombinant proteins from the zebrafish AK2 N-terminal domain, the antibody is typically validated for specificity and sensitivity in zebrafish-derived samples. Its application advances understanding of conserved molecular mechanisms across vertebrates and potential therapeutic targets for AK2-related pathologies.
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