| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, RPB33, DNA-directed RNA polymerase II subunit C, RPB31, POLR2C |
| Entrez GeneID | 5432 |
| WB Predicted band size | 31.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POLR2C抗体的模拟参考文献示例(仅供参考，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*"RNA Polymerase II Subunit RPB3 (POLR2C) as a Novel Therapeutic Target in Cancer"*
**作者**：Wang, L. et al.
**摘要**：本研究利用POLR2C特异性抗体，通过免疫组化和Western blot分析，发现POLR2C在多种实体瘤中异常高表达，并验证其通过调控转录延伸促进肿瘤细胞增殖的机制。
2. **文献名称**：*"Structural and Functional Analysis of the RNA Polymerase II Core Subunit Complex"*
**作者**：Garcia-Martinez, J. et al.
**摘要**：通过POLR2C抗体的免疫共沉淀(Co-IP)和冷冻电镜技术，揭示了RNA聚合酶II亚基间的相互作用网络，强调了POLR2C在维持复合体稳定性中的关键作用。
3. **文献名称**：*"Antibody-Based Profiling of RNA Polymerase II Phosphorylation and Subunit Composition"*
**作者**：Kim, H. & Buratowski, S.
**摘要**：开发了一种基于POLR2C抗体的ChIP-seq方法，用于定位全基因组范围内RNA聚合酶II的结合位点，并分析其磷酸化状态对转录周期的影响。
4. **文献名称**：*"Validation of Commercial Antibodies for Specific Detection of RNA Polymerase II Subunits"*
**作者**：Müller, F. et al.
**摘要**：系统评估了市售POLR2C抗体的特异性及适用性，验证其在流式细胞术和免疫荧光中的可靠性，为转录机制研究提供实验工具支持。
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**建议**：实际文献可通过PubMed、Web of Science等平台以关键词“POLR2C antibody”或“RNA polymerase II subunit antibody”检索，并筛选涉及功能研究、抗体开发或疾病相关应用的论文。
The POLR2C antibody targets the RNA polymerase II subunit C (POLR2C), a crucial component of the RNA polymerase II (Pol II) enzyme responsible for transcribing protein-coding genes in eukaryotes. Encoded by the POLR2C gene, this 33 kDa subunit (also known as RPB3) forms part of the Pol II core structure alongside subunits like POLR2A and POLR2B. Pol II is essential for mRNA synthesis, playing a central role in gene expression regulation. The POLR2C subunit contributes to polymerase assembly, stability, and recruitment to transcription initiation sites, interacting with transcription factors and coactivators.
POLR2C antibodies are widely used in research to study Pol II function, transcription mechanisms, and dysregulation in diseases. They enable detection of Pol II in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). In cancer biology, POLR2C is of interest due to its role in cell proliferation; aberrant Pol II activity is linked to tumorigenesis, making it a potential biomarker or therapeutic target. These antibodies also help investigate transcription inhibition by drugs or environmental stressors. Validated for specificity, POLR2C reagents are critical tools in molecular biology, supporting advances in understanding transcriptional regulation and its implications in health and disease.
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