| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, Cdk4 |
| Entrez GeneID | 94201 |
| WB Predicted band size | 33.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Rat Cdk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-303 amino acids from the C-terminal region of rat Cdk4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDK4抗体的3篇代表性文献示例(内容为模拟概括，建议通过学术数据库进一步验证)：
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1. **文献名称**：*Development and Validation of a Monoclonal Antibody Specific for CDK4 in Human Cancers*
**作者**：Smith J, et al.
**摘要**：本研究报道了一种高特异性抗人CDK4单克隆抗体的开发。通过免疫印迹和免疫组化验证，该抗体可特异性识别CDK4蛋白，并在乳腺癌和肺癌组织中检测到CDK4过表达，为癌症诊断提供了可靠工具。
2. **文献名称**：*CDK4 Expression in Melanoma: A Comparative Study Using Immunohistochemistry*
**作者**：Brown T, Lee R.
**摘要**：通过比较多种CDK4抗体的性能，作者确定了在黑色素瘤中检测CDK4表达的最优抗体。研究发现CDK4高表达与肿瘤进展相关，提示其作为预后标志物的潜力。
3. **文献名称**：*Targeting CDK4/6 in Breast Cancer: Antibody-Based Therapeutic Strategies*
**作者**：Zhang L, et al.
**摘要**：该文献探讨了抗CDK4抗体联合CDK4/6抑制剂在乳腺癌治疗中的作用。实验显示，抗体可增强抑制剂疗效，并通过阻断细胞周期进程抑制肿瘤生长。
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**建议**：可通过PubMed或Google Scholar搜索关键词“CDK4 antibody validation”、“CDK4 immunohistochemistry cancer”等获取真实文献。注意核对抗体货号或克隆号以确保应用场景匹配。
The R Cdk4 antibody is a laboratory-generated tool designed to detect and study cyclin-dependent kinase 4 (Cdk4), a serine/threonine kinase critical for cell cycle regulation. Cdk4 partners with cyclin D to drive G1-to-S phase progression by phosphorylating the retinoblastoma (Rb) protein, thereby releasing E2F transcription factors to activate genes required for DNA replication. Dysregulation of Cdk4. through overexpression or activating mutations, is implicated in cancers such as melanoma, breast cancer, and lymphoma. The "R" in R Cdk4 antibody typically denotes its recombinant origin or rabbit-derived monoclonal nature, ensuring high specificity and batch-to-batch consistency.
This antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to quantify Cdk4 expression, localization, and activity in cells or tissues. It aids in studying cell cycle mechanisms, cancer biology, and therapeutic responses, particularly in the context of Cdk4/6 inhibitors like palbociclib, which are FDA-approved for certain cancers. Researchers also employ R Cdk4 antibodies to validate genetic models (e.g., CRISPR/Cas9 knockouts) or assess biomarker status in clinical samples. Quality validation often includes testing on positive/negative cell lines and verifying molecular weight (~34 kDa) to confirm target specificity. Its role in both basic research and translational studies underscores its importance in advancing oncology and drug development.
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