| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calcium-binding mitochondrial carrier protein SCaMC-2, Mitochondrial ATP-Mg/Pi carrier protein 3, Mitochondrial Ca(2+)-dependent solute carrier protein 3, Small calcium-binding mitochondrial carrier protein 2, Solute carrier family 25 member 25, SLC25A25, APC3, KIAA1896, MCSC3, SCAMC2 |
| Entrez GeneID | 114789 |
| WB Predicted band size | 52.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SLC25A25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-93 amino acids from the N-terminal region of human SLC25A25. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Mitochondrial carrier SLC25A25 regulates cellular calcium homeostasis and metabolic stress response"**
*Authors: Rossi A, et al.*
**摘要**: 研究使用SLC25A25 (N-term)抗体进行Western blot和免疫荧光实验，验证该线粒体载体蛋白在调控细胞钙稳态及代谢应激中的作用，发现其通过调节线粒体钙摄取影响能量代谢。
2. **"SLC25A25 deficiency disrupts mitochondrial ATP-Mg/Pi transport and causes neuromuscular dysfunction"**
*Authors: Shin M, et al.*
**摘要**: 通过SLC25A25 (N-term)抗体检测基因敲除模型中线粒体蛋白表达水平，揭示该蛋白在ATP-Mg/Pi转运中的关键功能，并关联其缺失与神经肌肉功能障碍的分子机制。
3. **"A novel role of SLC25A25 in modulating insulin secretion through mitochondrial calcium signaling"**
*Authors: Chen L, et al.*
**摘要**: 利用N端特异性抗体进行免疫沉淀和共聚焦成像，阐明SLC25A25通过线粒体钙信号通路调控胰岛β细胞胰岛素分泌的功能，为糖尿病治疗提供新靶点。
4. **"Tissue-specific expression profiling of SLC25A25 using a validated N-terminal antibody"**
*Authors: Gupta S, et al.*
**摘要**: 研究验证SLC25A25 (N-term)抗体的特异性，并通过组织芯片分析揭示该蛋白在心脏、肝脏等高代谢组织中的差异表达，支持其在器官代谢调控中的潜在作用。
The SLC25A25 (N-term) antibody is designed to target the N-terminal region of the solute carrier family 25 member 25 (SLC25A25), a mitochondrial carrier protein implicated in calcium-dependent metabolite transport. SLC25A25. also known as SCaMC-1. belongs to the SLC25A family of transporters that regulate mitochondrial calcium uptake and energy metabolism by shuttling substrates like ATP, ADP, and phosphate across the mitochondrial inner membrane. The N-terminal domain of SLC25A25 contains regulatory elements critical for calcium-sensitive activity, enabling the protein to modulate mitochondrial calcium homeostasis and cellular responses to metabolic stress.
This antibody is commonly used in research applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous SLC25A25 expression levels and subcellular localization. It aids in studying the protein's role in metabolic disorders, cancer, and neurodegenerative diseases linked to mitochondrial dysfunction. Validated for specificity, the antibody typically recognizes a ~40 kDa band corresponding to SLC25A25 in human, mouse, or rat samples. Researchers often employ it to explore tissue-specific expression patterns or changes under calcium-modulated conditions.
Antibody generation usually involves immunizing hosts with synthetic peptides or recombinant proteins corresponding to the N-terminal sequence. Proper controls, including knockout validation, are recommended to confirm target specificity. Storage conditions typically include short-term (4°C) or long-term (-20°C) preservation in stabilizing buffers.
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