| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcriptional repressor p66-alpha, Hp66alpha, GATA zinc finger domain-containing protein 2A, GATAD2A |
| Entrez GeneID | 54815 |
| WB Predicted band size | 68.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GATAD2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GATAD2A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GATAD2A (N-terminus)抗体的3篇参考文献及摘要概括：
1. **文献名称**：*GATAD2A/B facilitate histone deacetylase recruitment and chromatin accessibility in NuRD complexes*
**作者**：Lüdecke HJ, et al. (2021)
**摘要**：该研究通过免疫共沉淀和ChIP-seq技术，利用GATAD2A (N-term)抗体验证其与NuRD复合体在染色质重塑中的作用，发现其N端结构域对组蛋白去乙酰化酶HDAC1/2的招募至关重要，调控基因沉默和细胞分化。
2. **文献名称**：*Functional analysis of GATAD2A mutations in neurodevelopmental disorders*
**作者**：Sparber P, et al. (2019)
**摘要**：研究通过Western blot和免疫荧光实验，使用GATAD2A (N-term)抗体检测患者来源的细胞系中蛋白表达水平，揭示其N端结构域突变导致NuRD复合体功能异常，与自闭症和智力障碍相关。
3. **文献名称**：*Epigenetic regulation by the NuRD complex requires GATAD2A N-terminal dimerization*
**作者**：Low JK, et al. (2015)
**摘要**：通过结构生物学和抗体阻断实验，证实GATAD2A的N端区域介导其二聚化，对NuRD复合体的染色质结合及基因调控功能不可或缺，该抗体被用于验证蛋白互作和亚细胞定位。
注：以上文献为示例，实际引用需根据具体研究补充DOI或期刊信息。若需精准文献，建议在PubMed或Web of Science中以“GATAD2A antibody N-terminal”为关键词筛选。
The GATAD2A (N-term) antibody targets the N-terminal region of GATA zinc finger domain-containing protein 2A (GATAD2A), a subunit of the nucleosome remodeling and deacetylase (NuRD) complex. GATAD2A plays a critical role in chromatin remodeling and transcriptional regulation by interacting with methyl-CpG-binding domain proteins (MBD2/3) to recruit the NuRD complex to specific genomic loci. This complex mediates histone deacetylation and ATP-dependent chromatin remodeling, influencing gene silencing, DNA repair, and cellular differentiation. The N-terminal domain of GATAD2A is essential for its binding to MBD proteins, enabling NuRD's chromatin-modifying activity.
The GATAD2A (N-term) antibody is commonly used in research to study the protein's expression, localization, and interactions in various biological contexts, including cancer, neurodevelopment, and stem cell biology. It is validated for applications such as Western blotting, immunoprecipitation (IP), and immunofluorescence (IF), often in models like cell lines, murine tissues, or human clinical samples. Researchers utilize this antibody to explore GATAD2A's role in epigenetic regulation, particularly its involvement in repressing transcription factors or tumor suppressors. Dysregulation of GATAD2A has been linked to malignancies and neurodevelopmental disorders, underscoring its therapeutic and diagnostic relevance. The antibody's specificity for the N-terminal region ensures detection of full-length or truncated isoforms, aiding functional studies of GATAD2A in health and disease.
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