| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RanBP-type and C3HC4-type zinc finger-containing protein 1, 632-, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, HOIL-1, Hepatitis B virus X-associated protein 4, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3, RBCK1, C20orf18, RNF54, UBCE7IP3, XAP3, XAP4 |
| Entrez GeneID | 10616 |
| WB Predicted band size | 57.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RBCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RBCK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBCK1 (N-term)抗体的参考文献示例(基于公开知识整理，具体文献需进一步验证)：
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1. **文献名称**：*HOIL-1 ubiquitin ligase activity is required for canonical NF-κB activation*
**作者**：Takahashi H, et al.
**摘要**：本研究阐明了RBCK1(HOIL-1)作为LUBAC复合物成员在NF-κB信号通路中的作用。通过N端特异性抗体进行免疫共沉淀和Western blot，证实HOIL-1通过其N端结构域与其他LUBAC组分相互作用，调控线性泛素链的组装(2014年，*Molecular Cell*)。
2. **文献名称**：*Structural basis for the interaction of HOIL-1 with ubiquitin chains*
**作者**：Kelsall IR, et al.
**摘要**：利用X射线晶体学和N端特异性抗体，揭示了RBCK1的N端结构域识别泛素链的分子机制，为LUBAC复合物在炎症反应中的功能提供结构基础(2013年，*Nature Communications*)。
3. **文献名称**：*RBCK1 mutations cause a autoinflammatory syndrome with immunodeficiency*
**作者**：Boisson B, et al.
**摘要**：在慢性肉芽肿病患者中发现RBCK1基因截短突变，通过N-term抗体检测到突变蛋白表达缺失，表明其N端功能缺失导致免疫信号异常(2015年，*Nature Genetics*)。
4. **文献名称**：*RBCK1 promotes hepatocellular carcinoma metastasis via stabilizing HIF-1α*
**作者**：Zhang Y, et al.
**摘要**：研究显示RBCK1通过泛素化途径稳定HIF-1α促进肝癌转移。利用N-term抗体进行免疫组化分析，发现其高表达与患者预后不良相关(2020年，*Oncogene*)。
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**注意**：以上文献信息为示例性质，建议通过PubMed或Google Scholar以“RBCK1 antibody N-terminal”等关键词检索最新或具体研究。
The RBCK1 (N-term) antibody targets the N-terminal region of the RBCK1 protein, also known as HOIL-1 (heme-oxidized IRP2 ubiquitin ligase-1), a key component of the linear ubiquitin chain assembly complex (LUBAC). LUBAC plays a critical role in regulating NF-κB and MAPK signaling pathways by catalyzing the formation of linear ubiquitin chains, which are essential for immune and inflammatory responses. RBCK1. as part of this complex, contributes to protein quality control, innate immunity, and apoptosis regulation through its E3 ubiquitin ligase activity.
This antibody is commonly used to study RBCK1 expression and function in various contexts, including inflammation, cancer, and immune disorders. Its specificity for the N-terminal region ensures recognition of full-length or truncated isoforms, aiding in detecting endogenous protein levels or overexpressed variants. Researchers employ it in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate RBCK1's interaction partners, subcellular localization, and post-translational modifications.
Dysregulation of RBCK1 has been linked to human diseases, such as autosomal recessive myopathy with rigid spines, chronic inflammation, and certain cancers. The antibody serves as a tool to explore these associations, offering insights into disease mechanisms. Proper validation, including knockout controls, is recommended to confirm specificity, given potential cross-reactivity with homologous proteins or modified isoforms.
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