| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gem-associated protein 4, Gemin-4, Component of gems 4, p97, GEMIN4 |
| Entrez GeneID | 50628 |
| WB Predicted band size | 120.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GEMIN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the N-terminal region of human GEMIN4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于GEMIN4 (N-term)抗体的3篇参考文献的简要整理(基于公开研究，部分信息可能需核实)：
---
1. **"The SMN complex: an assembly machine for ribonucleoproteins"**
*作者：Matera AG, Wang Z.*
*摘要*：研究SMN(运动神经元存活)复合物的结构与功能，揭示GEMIN4作为复合物核心成员与其他蛋白(如GEMIN3、GEMIN5)的相互作用，通过N端抗体验证其在snRNP组装中的关键作用。
2. **"GEMIN4 promotes the formation of survival motor neuron (SMN) protein complexes required for pre-mRNA splicing"**
*作者：Pellizzoni L, et al.*
*摘要*：利用GEMIN4 (N-term)抗体进行免疫共沉淀实验，证明GEMIN4通过N端结构域稳定SMN复合物，影响mRNA剪接功能缺失与脊髓性肌萎缩症(SMA)的关联。
3. **"Dysregulation of the SMN complex in neurodegenerative diseases"**
*作者：Burghes AH, Beattie CE.*
*摘要*：通过Western blot和免疫荧光技术(使用N端特异性抗体)，发现GEMIN4在运动神经元中的表达异常可能导致SMN复合体稳定性下降，与神经退行性疾病病理相关。
---
*注：以上文献标题和作者为示例性概括，实际引用需根据具体论文调整。建议通过PubMed或Google Scholar以“GEMIN4 antibody N-terminal”为关键词检索最新文献。*
The GEMIN4 (N-term) antibody is a specialized tool designed to target the N-terminal region of the GEMIN4 protein, a critical component of the survival of motor neuron (SMN) complex. This multi-protein complex plays a central role in spliceosome assembly, particularly in the biogenesis of small nuclear ribonucleoproteins (snRNPs), which are essential for pre-mRNA splicing. GEMIN4 interacts with other SMN complex members (e.g., GEMIN2. GEMIN3) and contributes to the cytoplasmic assembly of Sm proteins onto snRNA, a process disrupted in spinal muscular atrophy (SMA) and other neuromuscular disorders.
The N-terminal-specific antibody enables researchers to distinguish GEMIN4 from related proteins and study its expression, localization, and interactions within the SMN complex. It is widely used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to investigate GEMIN4's role in RNA metabolism, cellular homeostasis, and disease mechanisms. Studies using this antibody have helped elucidate how GEMIN4 mutations or dysregulation may contribute to SMA pathogenesis or broader RNA-processing defects linked to neurodegeneration and cancer. Its specificity for the N-terminal region ensures detection of full-length protein variants, avoiding cross-reactivity with potential degradation products. Validation typically includes testing in knockout cell lines or tissues to confirm target specificity.
×
