| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phospholipid-transporting ATPase IK, ATPase class I type 8B member 3, ATP8B3, ATP1K, FOS37502_2 |
| Entrez GeneID | 148229 |
| WB Predicted band size | 146.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ATP8B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the N-terminal region of human ATP8B3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ATP8B3(N-term)抗体的参考文献示例(注：文献为虚构示例，实际文献需通过学术数据库检索确认)：
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1. **标题**: *ATP8B3 N-terminal antibody validation and its role in sperm membrane remodeling*
**作者**: Smith A, et al.
**摘要**: 本研究验证了针对ATP8B3蛋白N端结构域的多克隆抗体的特异性，通过免疫印迹和免疫荧光证实其在人类精子中的定位。研究发现ATP8B3通过调节磷脂翻转参与精子顶体反应。
2. **标题**: *Characterization of a novel monoclonal antibody targeting ATP8B3 for functional studies in male infertility*
**作者**: Lee JH, et al.
**摘要**: 开发了一种抗ATP8B3 N端的单克隆抗体，用于检测睾丸组织及精子中的表达。实验表明ATP8B3缺失导致小鼠精子活力下降，提示其与男性不育相关。
3. **标题**: *ATP8B3 phospholipid transport activity revealed by N-terminal epitope-specific antibodies*
**作者**: Garcia R, et al.
**摘要**: 利用N端抗体研究ATP8B3的脂质转运功能，发现其通过维持膜磷脂不对称性影响精子获能，抗体阻断实验进一步证实了其酶活性的关键作用。
4. **标题**: *Immunohistochemical analysis of ATP8B3 expression in murine reproductive tissues*
**作者**: Chen X, et al.
**摘要**: 通过N-term抗体系统分析ATP8B3在小鼠睾丸和附睾中的时空表达模式，揭示其在精子成熟阶段的动态表达变化。
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建议通过PubMed或Google Scholar以关键词“ATP8B3 antibody N-terminal”检索真实文献，或查询抗体供应商(如Abcam、Santa Cruz)的产品引用文献列表获取具体信息。
The ATP8B3 (N-term) antibody is a targeted immunological tool designed to detect the N-terminal region of the ATP8B3 protein, a member of the P4-type ATPase family. ATP8B3. also known as ATPase phospholipid transporting 8B3. plays a critical role in maintaining phospholipid asymmetry in cell membranes by translocating phosphatidylserine and phosphatidylethanolamine from the outer to the inner leaflet. This enzyme is particularly implicated in sperm cell function and male fertility, with studies linking its dysfunction to reproductive disorders and certain cancers.
The antibody is commonly used in research to study ATP8B3 expression, localization, and regulatory mechanisms via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Its specificity for the N-terminal domain allows researchers to distinguish ATP8B3 from closely related isoforms and assess potential post-translational modifications or cleavage events. Validation typically includes testing in knockout cell lines or tissues to confirm minimal cross-reactivity. In reproductive biology, this antibody aids in exploring sperm maturation and acrosome formation, while in oncology, it helps investigate ATP8B3's potential role in cancer cell membrane dynamics and drug resistance. Proper controls are essential due to potential cross-reactivity with other P4-ATPases in experimental models.
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