| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase UBR1, 632-, N-recognin-1, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I, UBR1 |
| Entrez GeneID | 197131 |
| WB Predicted band size | 200.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This UBR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-274 amino acids from the N-terminal region of human UBR1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于UBR1 (N-term)抗体的3篇参考文献示例(注：文献为虚拟示例，仅供参考格式)：
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1. **"UBR1-mediated N-degron pathway regulates proteostasis in mammalian cells"**
*作者：Smith A, et al.*
**摘要**：研究利用UBR1 (N-term)抗体验证UBR1蛋白在细胞中的表达水平，发现UBR1通过识别N端降解信号调控错误折叠蛋白的泛素化及降解，影响细胞应激反应。
2. **"Role of UBR1 in Johanson-Blizzard syndrome: Insights from knockout models"**
*作者：Chen L, et al.*
**摘要**：通过UBR1 (N-term)抗体的免疫印迹分析，揭示UBR1基因突变导致蛋白功能丧失，引发胰腺发育异常，为Johanson-Blizzard综合征的分子机制提供证据。
3. **"UBR1 N-terminal domain is essential for substrate recognition in the ubiquitin-proteasome system"**
*作者：Yamamoto K, et al.*
**摘要**：使用UBR1 (N-term)抗体进行免疫共沉淀实验，证实UBR1的N端结构域直接结合底物，并解析其在泛素化过程中的底物特异性选择机制。
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**说明**：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science检索关键词(如“UBR1 antibody N-terminal”)获取真实文献。
The UBR1 (N-term) antibody targets the N-terminal region of UBR1 (Ubiquitin Protein Ligase E3 Component N-Recognin 1), a key E3 ubiquitin ligase in the N-end rule pathway, a conserved proteolytic system that marks specific proteins for degradation via the ubiquitin-proteasome system. UBR1 recognizes substrates bearing destabilizing N-terminal residues (e.g., bulky hydrophobic or basic residues) and facilitates their polyubiquitination, directing them to the 26S proteasome for breakdown. This pathway regulates diverse processes, including cell cycle progression, stress responses, and protein quality control.
UBR1 is notable for its role in developmental and metabolic homeostasis. Mutations in the UBR1 gene are linked to Johanson-Blizzard syndrome, a rare autosomal recessive disorder characterized by pancreatic insufficiency, growth delays, and congenital abnormalities. The UBR1 (N-term) antibody is widely used in research to study UBR1 expression, localization, and function through techniques like Western blotting, immunoprecipitation, and immunofluorescence. Its specificity for the N-terminal region ensures detection of full-length UBR1. helping distinguish it from truncated isoforms or degradation products. Validated in knockout controls, this antibody aids in elucidating UBR1’s contributions to proteostasis, disease mechanisms, and potential therapeutic targets.
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