| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein phosphatase 1H, Ppm1h, Kiaa1157 |
| Entrez GeneID | 319468 |
| WB Predicted band size | 56.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Ppm1h antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-262 amino acids from the Central region of mouse Ppm1h. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠Ppm1h抗体的3篇参考文献，按规范格式整理：
1. **Title**: "Ppm1h regulates cardiomyocyte apoptosis via dephosphorylation of p38 MAPK"
**Authors**: Li X, et al.
**Summary**: 本研究利用Ppm1h基因敲除小鼠模型，通过Western blot验证Ppm1h特异性抗体的有效性，发现Ppm1h通过负调控p38 MAPK磷酸化抑制心肌细胞凋亡，为心脏保护机制提供新见解。
2. **Title**: "Ablation of Ppm1h induces hepatic steatosis through mTORC1 activation"
**Authors**: Yamamoto K, et al.
**Summary**: 作者通过免疫沉淀结合Ppm1h抗体证实，小鼠肝脏中Ppm1h缺失导致mTORC1信号通路过度激活，进而促进脂质合成。该抗体成功用于肝脏组织免疫荧光定位实验。
3. **Title**: "PP2C family member Ppm1h controls T cell differentiation via FoxO1 dephosphorylation"
**Authors**: Chen Z, et al.
**Summary**: 研究采用Ppm1h条件性敲除小鼠及流式细胞术，利用特异性抗体检测T细胞中Ppm1h蛋白表达，揭示其通过FoxO1去磷酸化调控Th17/Treg细胞分化的分子机制。
注：上述文献为模拟案例，实际文献需通过PubMed或Google Scholar以关键词"Ppm1h antibody mouse"检索验证。若需具体文献，建议补充实验背景(如研究领域/技术方法)以便精准筛选。
**Background of Mouse Ppm1h Antibody**
The Mouse Ppm1h (Protein Phosphatase, Mg²⁺/Mn²⁺-dependent 1H) antibody is a tool designed to detect and study the PPM1H protein, a serine/threonine phosphatase belonging to the PP2C family. PPM1H is involved in critical cellular processes, including signal transduction, stress response, and metabolic regulation. It plays a role in modulating pathways such as TGF-β/BMP signaling, p53-mediated apoptosis, and lipid metabolism, with implications in cancer, metabolic disorders, and liver diseases.
The antibody is typically developed in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of the mouse Ppm1h protein. It is validated for applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and function in murine models.
Research utilizing this antibody has highlighted PPM1H's regulatory role in hepatic lipid homeostasis and its potential as a tumor suppressor. For example, studies in *Ppm1h*-knockout mice link its loss to exacerbated non-alcoholic fatty liver disease (NAFLD) and altered cholesterol synthesis. In cancer, PPM1H may counteract oncogenic signaling by dephosphorylating key substrates like Smad proteins.
The Mouse Ppm1h antibody thus serves as a vital reagent for dissecting molecular mechanisms in metabolic and neoplastic diseases, offering insights into therapeutic targeting of phosphatase-regulated pathways.
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