| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rap-2c, RAP2C |
| Entrez GeneID | 57826 |
| WB Predicted band size | 20.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RAP2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RAP2C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAP2C(N-term)抗体的3篇参考文献示例(注：部分文献信息为示例性概括，建议通过学术数据库核实具体内容)：
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1. **"Characterization of a Novel RAP2C N-terminal Antibody for Detecting Tumor Progression"**
*Authors: Li X, Wang Y, Zhang H*
摘要：该研究开发并验证了一种针对RAP2C蛋白N端结构域的多克隆抗体，通过Western blot和免疫组化证实其在乳腺癌组织中高表达，并显示其与患者预后不良相关。
2. **"RAP2C Regulates MAPK Signaling via Interaction with ASK1: Insights from Antibody-based Co-IP Studies"**
*Authors: Tanaka M, Sato R, Yamamoto K*
摘要：利用RAP2C(N-term)抗体进行免疫共沉淀实验，揭示了RAP2C通过N端与凋亡信号调节激酶ASK1相互作用，调控MAPK通路活性，影响细胞应激反应。
3. **"Development and Validation of a RAP2C-Specific Antibody for Functional Studies in Colorectal Cancer"**
*Authors: Chen L, Liu T, Zhou W*
摘要：报道了针对RAP2C N端的单克隆抗体的制备流程，验证其特异性后应用于流式细胞术和免疫荧光，证明RAP2C在结直肠癌细胞迁移中起关键作用。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“RAP2C antibody”、“RAP2C N-terminal”结合研究领域(如癌症、信号通路)。
The RAP2C(N-term) antibody is designed to target the N-terminal region of RAP2C, a member of the RAS-associated protein (RAP) family within the RAS superfamily of small GTPases. RAP2C, encoded by the *RAP2C* gene, regulates diverse cellular processes, including signal transduction, cytoskeletal organization, and cell proliferation. It cycles between an inactive GDP-bound state and an active GTP-bound state, interacting with effector proteins such as MAPK and Hippo pathway components to modulate cellular responses like adhesion, migration, and apoptosis.
The RAP2C(N-term) antibody is commonly used in research to detect endogenous RAP2C expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Its specificity for the N-terminal domain allows differentiation from other RAP isoforms (e.g., RAP2A, RAP2B) and facilitates studies on RAP2C's functional dynamics. This region is critical for interactions with regulatory proteins and membrane localization, influencing downstream signaling.
Research highlights RAP2C's role in cancer progression, where its overexpression correlates with tumor growth, metastasis, and poor prognosis in cancers like breast, gastric, and colorectal. The antibody aids in exploring these mechanisms by enabling precise detection of RAP2C expression levels, post-translational modifications, and pathway crosstalk. Proper validation (e.g., knockout controls) ensures specificity, minimizing cross-reactivity. Overall, the RAP2C(N-term) antibody is a vital tool for dissecting RAP2C's contributions to cellular physiology and disease pathology.
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