| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, Pik3r1 |
| Entrez GeneID | 18708 |
| WB Predicted band size | 83.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Pik3r1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the N-terminal region of mouse Pik3r1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Pik3r1 (N-term)抗体的3篇参考文献示例(注：文献为虚拟概括，具体引用需核实原始论文)：
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1. **文献名称**：*Regulation of PI3K signaling by the p85α subunit in metabolic tissues*
**作者**：Ueki K, et al.
**摘要**：研究通过抗Pik3r1 (N-term)抗体的Western blot分析，揭示了p85α亚基在胰岛素信号通路中的调控作用，并发现其表达异常与胰岛素抵抗相关。
2. **文献名称**：*Characterization of Pik3r1 knockout mice using N-terminal specific antibodies*
**作者**：Fruman DA, et al.
**摘要**：利用针对Pik3r1蛋白N端的特异性抗体，验证了基因敲除小鼠模型中p85α的缺失，并证明其在PI3K/AKT通路中的关键功能。
3. **文献名称**：*Role of p85α in liver regeneration and tumorigenesis*
**作者**：Jia S, et al.
**摘要**：通过免疫组化(使用抗Pik3r1 N端抗体)和分子实验，发现p85α在肝脏再生和肝癌发生中通过调控PI3K活性发挥作用。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“Pik3r1 antibody N-term”或“p85α PI3K antibody”，并筛选涉及小鼠模型或抗体应用的论文。部分商业抗体手册(如CST或Abcam)也可能提供相关引用文献。
The Mouse Pik3r1 (N-term) antibody is designed to detect the N-terminal region of the phosphatidylinositol 3-kinase regulatory subunit alpha (PI3K p85α), encoded by the *Pik3r1* gene in mice. This protein plays a critical role in the PI3K-AKT signaling pathway, regulating cellular processes such as growth, survival, metabolism, and apoptosis. The p85α subunit binds to the p110 catalytic subunit of PI3K, stabilizing it and modulating its lipid kinase activity. The N-terminal region of p85α contains SH3 and proline-rich domains, which mediate interactions with other signaling molecules (e.g., receptor tyrosine kinases, adaptor proteins), influencing pathway activation.
This antibody is widely used in research to study PI3K signaling dynamics in mouse models, particularly in contexts like cancer, diabetes, and immune disorders. By targeting the N-terminal region, it enables detection of full-length p85α and potentially distinct isoforms or cleavage products. Applications include Western blotting, immunohistochemistry, and immunoprecipitation to assess protein expression, localization, and interactions. Validation often involves knockout or knockdown controls to confirm specificity.
Dysregulation of *Pik3r1* is linked to insulin resistance, tumorigenesis, and aberrant cell proliferation, making this antibody a valuable tool for mechanistic studies and therapeutic target exploration. Its use supports investigations into how PI3K pathway mutations or altered expression contribute to disease pathogenesis.
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