| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1, Nephrin-like protein 1, KIRREL, KIRREL1, NEPH1 |
| Entrez GeneID | 55243 |
| WB Predicted band size | 83.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This KIRREL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505-534 amino acids from the C-terminal region of human KIRREL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是假设性示例的参考文献(非真实文献，仅作格式参考)：
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1. **"Characterization of KIRREL (NEPH1) C-terminal antibody in podocyte adhesion complexes"**
*作者：Tomoko Takano, et al.*
摘要：该研究验证了KIRREL C端抗体的特异性，证实其可用于检测内源性KIRREL在肾足细胞中的定位，并揭示其与nephrin的相互作用对裂孔膜结构的重要性。
2. **"Role of KIRREL in glomerular filtration barrier: Insights from antibody-based inhibition assays"**
*作者：Andrey Shaw, et al.*
摘要：通过C端抗体阻断KIRREL功能，发现其缺失导致足细胞骨架重组和蛋白尿，提示KIRREL在维持肾小球滤过屏障中的关键作用。
3. **"KIRREL C-terminal domain mediates signaling through MAPK pathway in diabetic nephropathy"**
*作者：Lawrence B. Holzman, et al.*
摘要：利用特异性抗体证实KIRREL的C端结构域在高糖环境下激活MAPK通路，可能与糖尿病肾病足细胞损伤相关。
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(注：以上为模拟内容，实际文献需通过PubMed等数据库检索关键词如“KIRREL antibody C-term”或“NEPH1 C-terminal epitope”获取。)
The KIRREL (C-term) antibody is a specialized immunological tool designed to target the C-terminal region of the KIRREL protein, also known as NEPH1 (Kin of IRRE-like protein 1). KIRREL is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, primarily recognized for its role in cell-cell adhesion and signaling. It is highly expressed in podocytes of the kidney glomerulus, where it interacts with nephrin and other slit diaphragm components to maintain the filtration barrier. Dysregulation of KIRREL has been implicated in renal disorders, including congenital nephrotic syndrome and proteinuria, as well as in cancer progression due to its involvement in cell migration and metastasis.
The C-terminal domain of KIRREL is critical for its intracellular signaling functions, mediating interactions with cytoskeletal adaptors like MAGI-1 and CD2AP. Antibodies targeting this region are widely used in Western blotting, immunofluorescence, and immunohistochemistry to study KIRREL expression, localization, and post-translational modifications. They have become essential in renal research, particularly for investigating glomerular injury mechanisms, podocyte biology, and congenital kidney diseases. Additionally, these antibodies aid in exploring KIRREL's oncogenic roles in malignancies, where aberrant expression may influence tumor cell invasiveness. Commercial KIRREL (C-term) antibodies are typically raised in rabbits or mice, with validation in specific experimental models to ensure specificity for research or diagnostic applications.
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