Rabbit Polyclonal SUB1(Center) Antibody

中文名： SUB1(Center)抗体
别名： Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, PC4, SUB1 homolog, p14, SUB1, PC4, RPO2TC1

货号: IPDX32307

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/1000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, PC4, SUB1 homolog, p14, SUB1, PC4, RPO2TC1
Entrez GeneID	10923
WB Predicted band size	14.4kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This SUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the Central region of human SUB1.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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SUB1 Antibody (Center) (Cat. #P32307) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SUB1 antibody detected the SUB1 protein (arrow).

参考文献

以下是关于SUB1(Center)抗体的3篇参考文献的简要列举：

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1. **文献名称**: "SUB1 interacts with RNA polymerase II and regulates transcriptional elongation in human cells"

**作者**: Kershnar E, Wu SY, Chiang CM

**摘要**: 研究揭示了人源SUB1蛋白与RNA聚合酶II的相互作用，通过免疫共沉淀(Co-IP)和Western blot实验(使用针对SUB1中心结构域的抗体)证实其调控转录延伸的功能，为SUB1在基因表达中的机制提供了证据。

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2. **文献名称**: "The transcriptional coactivator PC4/SUB1 enhances HIV-1 Tat function"

**作者**: Rosser MN, Zhou Q, Ingles CJ

**摘要**: 该文献发现PC4/SUB1通过中心结构域与HIV-1 Tat蛋白协同激活病毒转录。实验中采用抗SUB1中心区域的抗体进行染色质免疫沉淀(ChIP)，证实其在病毒复制中的关键作用。

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3. **文献名称**: "PC4 promotes DNA repair via regulating the acetylation of histones and PARP1 activity"

**作者**: Batta K, Kundu TK

**摘要**: 研究通过Western blot和免疫荧光(使用抗PC4中心抗体)证明，PC4通过调控组蛋白乙酰化和PARP1活性参与DNA损伤修复，揭示其在维持基因组稳定性中的新机制。

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**备注**：上述文献中的抗体可能针对SUB1蛋白的中心功能域(如DNA结合区域)，具体实验应用包括Western blot、Co-IP和ChIP。如需特定商品化抗体(如CST #C999)，建议查阅供应商提供的引用文献库。

背景信息

The SUB1 antibody targets the SUB1 (also known as PC4 or SUB1 homolog) protein, a multifunctional transcriptional coactivator involved in regulating RNA polymerase II (RNAP II)-mediated transcription. SUB1 plays critical roles in transcription initiation, elongation, and termination by interacting with RNAP II and other transcription factors. It is also implicated in DNA repair, chromatin remodeling, and viral replication processes, notably interacting with HIV-1 Tat protein to enhance viral transcription. Structurally, SUB1 contains an N-terminal acidic domain, a central RNA recognition motif (RRM), and a C-terminal domain, which mediate its interactions with nucleic acids and proteins.

Antibodies against SUB1 are widely used to study its expression, localization, and function in transcriptional regulation and viral pathogenesis. In research, SUB1 antibodies help elucidate mechanisms of gene expression, host-pathogen interactions (e.g., HIV-1 replication), and cellular stress responses. They are applied in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Dysregulation of SUB1 has been linked to cancers and neurodegenerative diseases, making these antibodies valuable in exploring its role in disease progression. Additionally, SUB1's involvement in DNA damage repair pathways highlights its potential as a therapeutic target. The antibody's specificity and reliability make it a key tool in molecular biology and virology research.