| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SF3b50, Spliceosome-associated protein 49, SAP 49, SF3B4, SAP49 |
| Entrez GeneID | 10262 |
| WB Predicted band size | 44.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SF3B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-187 amino acids from the Central region of human SF3B4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SF3B4抗体的3篇参考文献示例(内容为虚构，仅供参考格式)：
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1. **文献名称**：*SF3B4 Antibody Validation in Spliceosome Assembly Studies*
**作者**：Chen X, Li W, et al.
**摘要**：本研究利用抗SF3B4抗体通过免疫共沉淀(Co-IP)和质谱技术，揭示了SF3B4在剪接体动态组装中的关键作用，并验证了抗体在检测剪接体复合物中的特异性。
2. **文献名称**：*SF3B4 Expression Correlates with Poor Prognosis in Gastric Cancer*
**作者**：Yamamoto S, et al.
**摘要**：通过免疫组化(IHC)和Western blot分析，该研究证实SF3B4在胃癌组织中高表达，且其表达水平与患者生存率显著相关，抗体的特异性通过siRNA敲降实验验证。
3. **文献名称**：*Functional Analysis of SF3B4 Mutations Using CRISPR and Antibody-Based Assays*
**作者**：Garcia-Ruiz A, López-Otín C
**摘要**：结合CRISPR基因编辑和抗SF3B4抗体的免疫荧光技术，研究发现SF3B4突变导致核仁定位异常，影响RNA剪接功能，为相关遗传疾病提供机制解释。
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*注：以上文献为示例，实际引用请通过学术数据库(如PubMed)检索真实发表的研究。*
The SF3B4 (Center) antibody is a specific immunological tool designed to detect the SF3B4 protein, a critical component of the spliceosome complex involved in pre-mRNA splicing. SF3B4. also known as SAP49. is a subunit of the SF3b complex within the U2 small nuclear ribonucleoprotein (snRNP), essential for recognizing branch site sequences during spliceosome assembly. This antibody typically targets a central region of the SF3B4 protein, enabling its use in applications like Western blotting, immunoprecipitation, and immunofluorescence to study spliceosome dynamics and RNA processing mechanisms.
Research utilizing the SF3B4 (Center) antibody has provided insights into its role in cellular processes, including gene expression regulation and genomic stability. Dysregulation of SF3B4 is linked to diseases such as cancer, particularly in malignancies with splicing factor mutations (e.g., myelodysplastic syndromes). The antibody aids in investigating SF3B4's functional interactions, post-translational modifications, and aberrant expression patterns in pathological contexts.
Validated for specificity and sensitivity, the SF3B4 (Center) antibody is crucial for studies exploring spliceosome biology, cancer mechanisms, and therapeutic targeting of splicing anomalies. Its reliability in distinguishing SF3B4 from related proteins ensures accurate analysis in both basic and translational research settings.
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