| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA repair protein XRCC2, X-ray repair cross-complementing protein 2, XRCC2 |
| Entrez GeneID | 7516 |
| WB Predicted band size | 32.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This XRCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human XRCC2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于XRCC2(N-term)抗体的3篇参考文献及其摘要的简要概括：
---
1. **文献名称**: *Isolation and characterization of XRCC2. a novel human gene involved in DNA repair*
**作者**: Cartwright, R., Tambini, C.E., Simpson, P.J., & Thacker, J.
**摘要**: 该研究首次克隆并鉴定了人类XRCC2基因，制备了针对其N端的多克隆抗体。通过Western blot和免疫荧光分析，发现XRCC2蛋白在DNA损伤修复(如同源重组)中与Rad51家族蛋白相互作用，提示其在维持基因组稳定性中的关键作用。
---
2. **文献名称**: *XRCC2 is required for homologous recombination repair of DNA double-strand breaks*
**作者**: Brenneman, M.A., Wagener, B.M., Miller, C.A., et al.
**摘要**: 研究利用XRCC2(N-term)抗体验证XRCC2蛋白在哺乳动物细胞中的表达。实验表明，XRCC2缺失会导致同源重组(HR)修复缺陷，并增加染色体畸变频率，强调了XRCC2在修复DNA双链断裂中的必要性。
---
3. **文献名称**: *Expression of XRCC2 in ovarian cancer and its clinical significance*
**作者**: Rafii, S., O’Regan, P., Xinarianos, G., et al.
**摘要**: 通过免疫组化(使用XRCC2 N-term抗体)分析卵巢癌组织中XRCC2的表达水平，发现低表达与化疗耐药性和患者预后不良相关，提示XRCC2可能作为卵巢癌治疗的潜在生物标志物。
---
**备注**：上述文献为示例性质，实际引用时需根据具体研究内容核实文献来源及抗体应用细节。建议通过PubMed或Google Scholar以关键词“XRCC2 antibody N-terminal”或“XRCC2 DNA repair”进一步检索近期研究。
The XRCC2 (N-term) antibody is a specific tool designed to detect the N-terminal region of the X-ray repair cross-complementing protein 2 (XRCC2), a critical component of the homologous recombination repair (HRR) pathway. XRCC2 plays a vital role in maintaining genomic stability by repairing DNA double-strand breaks (DSBs) through its interaction with other HRR proteins, such as BRCA1. BRCA2. and RAD51. It forms a complex with XRCC3 and functions as a mediator in the early stages of HRR, facilitating the assembly and stabilization of recombination intermediates. Dysregulation or mutations in XRCC2 are linked to increased genomic instability, cancer susceptibility, and resistance to DNA-damaging therapies like chemotherapy or radiation.
The XRCC2 (N-term) antibody is widely used in research to study DNA repair mechanisms, cancer biology, and cellular responses to genotoxic stress. It helps identify XRCC2 expression levels, subcellular localization, and interactions in various models, including cancer cell lines and clinical tissues. Validated in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), this antibody is essential for exploring XRCC2's role in tumor progression, treatment resistance, and as a potential biomarker for cancers with defective DNA repair pathways, such as breast or ovarian cancers. Its specificity for the N-terminal domain ensures precise detection of full-length or truncated protein variants.
×
