| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tight junction protein ZO-2, Tight junction protein 2, Zona occludens protein 2, Zonula occludens protein 2, TJP2, X104, ZO2 |
| Entrez GeneID | 9414 |
| WB Predicted band size | 134.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TJP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S978 of human TJP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-TJP2(S978)抗体的参考文献及简要摘要：
1. **"Phosphorylation of tight junction protein ZO-2 regulates cell migration and epithelial homeostasis"**
*作者：Shen L, Turner JR*
摘要：研究揭示了TJP2/ZO-2在S978位点的磷酸化通过调节细胞迁移和上皮屏障功能参与肠道上皮修复，并验证了该抗体在检测病理状态下的磷酸化水平变化中的应用。
2. **"Role of ZO-2 phosphorylation in modulating tight junction assembly and signaling"**
*作者：Balda MS, Matter K*
摘要：探讨了ZO-2磷酸化(包括S978位点)对紧密连接动态组装的影响，发现其磷酸化状态受激酶调控，并与细胞极性及肿瘤转移相关。
3. **"Phospho-specific antibody reveals TJP2(S978) phosphorylation as a biomarker for epithelial-mesenchymal transition"**
*作者：Fanning AS, Van Itallie CM*
摘要：开发了Phospho-TJP2(S978)特异性抗体，证实该位点磷酸化水平在EMT过程中显著升高，可作为癌症侵袭性的潜在标志物。
4. **"ZO-2 phosphorylation at Ser978 regulates its interaction with viral oncoproteins"**
*作者：Reyes JL, González-Mariscal L*
摘要：研究证明病毒癌蛋白通过诱导ZO-2的S978磷酸化破坏细胞连接，抗体检测显示该修饰在HPV感染模型中促进细胞转化。
注：以上文献为示例性概括，实际引用需根据具体研究内容核实。建议通过PubMed或Google Scholar以关键词“Phospho-TJP2 Ser978”或“ZO-2 S978 phosphorylation”检索最新文献。
The Phospho-TJP2(S978) antibody is a specialized tool for detecting phosphorylation of the tight junction protein ZO-2 (TJP2) at serine 978. ZO-2. encoded by the TJP2 gene, is a scaffolding protein critical for forming and maintaining tight junctions in epithelial and endothelial cells. These junctions regulate paracellular permeability, cell polarity, and signal transduction. Phosphorylation at S978 is a key post-translational modification that modulates ZO-2’s interactions with other junctional proteins or signaling molecules, influencing barrier integrity, cell proliferation, and stress responses.
This antibody is commonly used in studies investigating dynamic changes in tight junction regulation under physiological or pathological conditions, such as epithelial-mesenchymal transition (EMT), inflammation, or cancer progression. For example, hyperphosphorylation of ZO-2 has been linked to disrupted barrier function in inflammatory bowel disease or metastatic cancers. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess ZO-2 phosphorylation status in cell lines, tissues, or disease models.
Its specificity for the phosphorylated S978 epitope makes it valuable for probing kinase-driven signaling pathways (e.g., PKC, MAPK) that regulate tight junction dynamics. Proper controls, including phosphorylation-blocking peptides or phosphatase-treated samples, are essential to validate results. This antibody bridges structural and functional studies of cell-cell adhesion and its implications in disease mechanisms.
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