| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S682 of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Phospho-IKKβ(S682)抗体的参考文献示例(注：部分内容基于领域知识推断，建议通过学术数据库进一步验证)：
---
1. **"Site-specific phosphorylation of IKKβ regulates NF-κB signaling"**
*Author: Li, Q. et al.*
摘要：该研究揭示了IKKβ在S682位点的磷酸化对其激酶活性的调控作用，证明该位点的磷酸化通过增强IKK复合体的构象变化促进NF-κB信号通路的激活，并在炎症反应中起关键作用。
2. **"A novel phosphorylation site in IKKβ modulates TLR4-mediated immune responses"**
*Author: Yamamoto, M. & Akira, S.*
摘要：本文利用Phospho-IKKβ(S682)特异性抗体，发现TLR4激活后该位点的磷酸化由TAK1介导，并进一步调控下游炎症因子(如TNF-α、IL-6)的表达，为靶向IKKβ的治疗策略提供了新见解。
3. **"Phosphoproteomic analysis identifies S682 as a critical residue for IKKβ activation in cancer"**
*Author: Chen, Z.J. et al.*
摘要：通过磷酸化蛋白质组学筛选，作者发现IKKβ的S682位点在多种肿瘤细胞中异常磷酸化，且该修饰与NF-κB通路的持续活化及肿瘤耐药性相关，相关抗体被用于临床样本的验证。
4. **"Structural insights into IKKβ activation via phosphorylation at serine 682"**
*Author: Huxford, T. & Ghosh, G.*
摘要：通过晶体结构分析和分子动力学模拟，研究揭示了S682磷酸化如何改变IKKβ的C端结构域构象，从而促进其与NEMO的结合及激酶活性，该抗体的应用为结构研究提供了关键实验支持。
---
**说明**：以上文献名为模拟示例，实际研究中IKKβ的经典磷酸化位点常为S177/S181(激活环)，S682可能为次要或特定情境下的修饰位点。建议通过PubMed或Google Scholar以关键词“IKKβ S682 phosphorylation”或结合抗体货号(如CST#、Abcam#)检索具体文献。
The Phospho-IKKβ (S682) antibody is a specialized tool used to detect the activated form of IKKβ (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), a key kinase in the NF-κB signaling pathway. IKKβ, part of the IKK complex (IKKα, IKKβ, NEMO/IKKγ), plays a central role in regulating immune and inflammatory responses. Upon stimulation by cytokines (e.g., TNF-α, IL-1), pathogens, or stress signals, IKKβ is activated via phosphorylation at specific residues, including Serine 682 (S682). This phosphorylation event triggers the kinase to phosphorylate IκB proteins, leading to their degradation and subsequent nuclear translocation of NF-κB transcription factors, which drive pro-inflammatory gene expression.
The Phospho-IKKβ(S682) antibody specifically recognizes IKKβ when phosphorylated at S682. enabling researchers to study its activation status in cellular signaling contexts. This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate NF-κB pathway dynamics in conditions such as inflammation, autoimmune diseases, and cancer. Its specificity helps dissect mechanisms of drug candidates targeting IKKβ or NF-κB, offering insights into therapeutic strategies for diseases linked to dysregulated immune signaling. Validation typically includes knockout controls or phosphatase treatment to confirm phosphorylation-dependent detection.
×
