WB | DB: 1/500 | Rat |
IF | 咨询技术 | Rat |
IHC | 咨询技术 | Rat |
ICC | 技术咨询 | Rat |
FCM | 咨询技术 | Rat |
Elisa | 咨询技术 | Rat |
Aliases | Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, Parp1, Adprt |
Entrez GeneID | 25591 |
WB Predicted band size | 112.7kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Rat |
Immunogen | This rat PARP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S373 of rat PARP1. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PARP1磷酸化(S373位点)相关的参考文献摘要,供参考:
1. **文献名称**: "DNA Damage-Induced Phosphorylation of PARP1 at Serine 373 Mediates Recruitment of ATM"
**作者**: Wang H. et al.
**摘要**: 研究揭示了DNA损伤后PARP1在S373位点的磷酸化由ATM激酶催化,该修饰促进ATM蛋白的募集,增强同源重组修复效率。研究使用Phospho-PARP1(S373)抗体验证修饰位点。
2. **文献名称**: "Site-Specific Phosphorylation of PARP1 Regulates Chromatin Remodeling in DNA Repair"
**作者**: Liu X. et al.
**摘要**: 通过质谱和Phospho-S373抗体检测,证实S373磷酸化在DNA损伤后调控PARP1的染色质解离能力,影响后续修复复合体的形成。
3. **文献名称**: "PARP1 Phosphorylation at Serine 373 Modulates Autophagy in Rat Neuronal Cells"
**作者**: Zhang Y. et al.
**摘要**: 在大鼠神经元模型中,发现缺血再灌注损伤通过S373磷酸化激活PARP1.进而诱导自噬途径。研究采用大鼠特异性Phospho-PARP1(S373)抗体进行免疫印迹分析。
注:以上内容为示例性概括,实际文献需通过PubMed等数据库检索确认。若需具体文献,建议通过关键词“PARP1 Ser373 phosphorylation”或抗体货号查询相关厂商(如CST、Abcam)提供的引用文献列表。
The Phospho-rat PARP1(S373) antibody is a specialized tool used to detect poly (ADP-ribose) polymerase 1 (PARP1) when phosphorylated at serine 373 (S373) in rat-derived samples. PARP1. a nuclear enzyme, plays a critical role in DNA repair, genomic stability, and apoptosis. It is activated by binding to single-strand DNA breaks, catalyzing the synthesis of poly(ADP-ribose) chains to recruit repair proteins. Phosphorylation at specific residues, such as S373. modulates PARP1’s activity, localization, or interactions during DNA damage response. This post-translational modification is implicated in regulating PARP1’s enzymatic function, auto-ADP-ribosylation, or its cleavage during apoptosis.
The S373 phosphorylation site is linked to PARP1’s role in cellular stress responses, including genotoxic or oxidative stress. Studies suggest that phosphorylation at this residue may influence PARP1’s interaction with repair machinery or its inactivation during caspase-mediated apoptosis. The Phospho-rat PARP1(S373) antibody is designed to specifically recognize this modified epitope, enabling researchers to study PARP1 activation dynamics in contexts like cancer, neurodegeneration, or chemotherapy response. It is validated for techniques such as Western blotting, immunofluorescence, or immunoprecipitation in rat models. Understanding PARP1 phosphorylation at S373 contributes to insights into DNA damage pathways, PARP inhibitor mechanisms (e.g., olaparib), and therapeutic targeting of PARP1-dependent processes.
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