Rabbit Polyclonal Phospho-IKKB(S705) Antibody

The Phospho-IKKβ (S705) antibody is a crucial tool for studying the activation status of the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ), a key component of the IKK complex in the NF-κB signaling pathway. IKKβ, primarily involved in phosphorylating the inhibitor of NF-κB (IκB), enables NF-κB translocation to the nucleus to regulate immune, inflammatory, and stress responses. Phosphorylation at serine 705 (S705) is associated with IKKβ activation, often triggered by pro-inflammatory cytokines (e.g., TNF-α, IL-1) or pathogens via pattern recognition receptors. This antibody specifically detects the phosphorylated form of IKKβ at S705. allowing researchers to monitor pathway activation in cellular models, tissues, or disease states like cancer, autoimmune disorders, or chronic inflammation. It is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Validation typically includes testing in knockout cells or stimulation/inhibition experiments to confirm specificity. Understanding IKKβ phosphorylation dynamics provides insights into therapeutic targeting of NF-κB-driven pathologies, including drug resistance mechanisms and inflammatory diseases.