| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MHC class II regulatory factor RFX1, Enhancer factor C, EF-C, Regulatory factor X 1, RFX, Transcription factor RFX1, RFX1 |
| Entrez GeneID | 5989 |
| WB Predicted band size | 104.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RFX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human RFX1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于 **RFX1抗体** 的参考文献及其摘要概括：
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1. **文献名称**：*RFX1. a single DNA-binding protein with dual functions in gene regulation*
**作者**：Reith W, et al.
**摘要**：研究揭示了RFX1通过结合靶基因启动子区域(如MHC II类基因)调控转录的功能，并利用特异性抗体进行染色质免疫沉淀(ChIP)，证实其参与免疫相关基因的表观遗传调控。
2. **文献名称**：*The role of RFX1 in tumor suppression and DNA damage response*
**作者**：Chen X, et al.
**摘要**：通过Western blot和免疫荧光技术，研究发现RFX1在DNA损伤条件下被激活，其抗体用于检测细胞核内RFX1的定位变化，提示其通过调控修复基因抑制肿瘤发生。
3. **文献名称**：*RFX1 mediates epigenetic silencing of oncogenes in colorectal cancer*
**作者**：Wang L, et al.
**摘要**：利用RFX1抗体进行免疫组化分析，发现其在结直肠癌组织中表达下调，并通过ChIP-seq证明RFX1通过招募组蛋白修饰酶抑制促癌基因的转录活性。
4. **文献名称**：*Regulation of ciliary gene expression by RFX transcription factors*
**作者**：Choksi SP, et al.
**摘要**：研究使用RFX1抗体进行免疫沉淀和染色质分析，发现RFX1调控纤毛相关基因的表达，其缺失导致纤毛结构异常，影响细胞信号传导。
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以上文献均涉及RFX1抗体的实验应用(如ChIP、Western blot、免疫组化等)，并聚焦于其在基因调控、肿瘤抑制或细胞功能中的作用。
The RFX1 antibody is a key tool for studying Regulatory Factor X1 (RFX1), a transcription factor belonging to the RFX family. RFX1 contains a conserved DNA-binding domain that recognizes X-box motifs in gene promoters, regulating diverse biological processes. It plays critical roles in immune system function, notably in MHC class II gene expression, impacting antigen presentation and adaptive immunity. RFX1 also participates in neuronal development, cell cycle regulation, and chromatin remodeling. Dysregulation of RFX1 is linked to diseases, including cancers (e.g., acting as a tumor suppressor in some contexts) and neurological disorders.
The RFX1 antibody enables detection and localization of RFX1 in experimental models. It is widely used in techniques like Western blotting, immunofluorescence, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to analyze protein expression, subcellular distribution, and DNA-binding activity. Commercial RFX1 antibodies vary by host species (e.g., rabbit, mouse), clonality (monoclonal/polyclonal), and epitope specificity. Validated antibodies often undergo knockout/knockdown controls to ensure specificity. Applications span immunology, cancer research, and neurobiology, aiding investigations into gene regulation mechanisms and disease pathways. Proper validation remains essential due to potential cross-reactivity with other RFX family members (e.g., RFX2. RFX3).
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