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Rabbit Polyclonal SF3B2 Antibody

  • 中文名: SF3B2抗体
  • 别    名: Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145 kDa subunit, SF3b145, SF3b150, Spliceosome-associated protein 145, SAP 145, SF3B2, SAP145
货号: IPDX31598
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSplicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145 kDa subunit, SF3b145, SF3b150, Spliceosome-associated protein 145, SAP 145, SF3B2, SAP145
Entrez GeneID10992
WB Predicted band size100.2kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenThis SF3B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539-568 amino acids from the Central region of human SF3B2.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于SF3B2抗体的3篇文献摘要概览(基于公开学术资源整理):

1. **文献名称**:*"SF3B2 as a novel cancer biomarker: Insights into spliceosome dysregulation in hepatocellular carcinoma"*

**作者**:Li, X. et al.

**摘要**:研究通过免疫组化(使用SF3B2特异性抗体)发现SF3B2在肝癌组织中高表达,且与患者预后不良相关,提示其可能通过调控异常剪接促进肿瘤进展。

2. **文献名称**:*"Structural and functional characterization of the SF3B complex in pre-mRNA splicing"*

**作者**:Cretu, C. et al.

**摘要**:利用SF3B2抗体进行免疫共沉淀和冷冻电镜分析,揭示了SF3B复合物(含SF3B2亚基)在剪接体组装中的关键作用,并解析其与pre-mRNA结合的分子机制。

3. **文献名称**:*"A pan-cancer analysis of SF3B2 mutations and their association with splicing defects"*

**作者**:Wang, Q. et al.

**摘要**:通过Western blot(基于SF3B2抗体)和RNA测序,发现SF3B2突变在多癌种中导致剪接异常,并与肿瘤微环境免疫逃逸相关。

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**注**:以上文献为示例,实际引用需查询具体数据库(如PubMed)。若需真实文献,建议使用关键词 **"SF3B2 antibody" + "spliceosome"/"cancer"** 在PubMed或Google Scholar中检索近年研究。

背景信息

The SF3B2 antibody is a crucial tool for studying the SF3B2 protein, a core component of the spliceosome complex responsible for pre-mRNA splicing. SF3B2. a subunit of the SF3B complex, plays a vital role in recognizing branch site sequences during spliceosome assembly, ensuring accurate intron removal and exon ligation. Dysregulation of SF3B2 has been linked to splicing errors implicated in cancers, such as myelodysplastic syndromes and chronic lymphocytic leukemia, particularly when associated with SF3B1 mutations. The antibody enables researchers to investigate SF3B2's expression, localization, and interactions within the spliceosome machinery. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess protein levels in cellular or tissue samples, study spliceosome dynamics, or validate SF3B2 knockdown/knockout models. Additionally, it aids in exploring SF3B2's role in disease mechanisms, including its potential as a biomarker or therapeutic target. Commercial SF3B2 antibodies are typically raised against specific epitopes (e.g., human SF3B2 fragments) and validated for specificity across applications. Recent studies also utilize these antibodies to probe the effects of splicing-modulating drugs or SF3B complex inhibitors in cancer models, underscoring their relevance in both basic research and translational oncology.

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