| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mesogenin-1, Paraxial mesoderm-specific mesogenin1, pMesogenin1, pMsgn1, MSGN1 |
| Entrez GeneID | 343930 |
| WB Predicted band size | 20.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MSGN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from the Central region of human MSGN1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MSGN1抗体的3篇参考文献及其摘要概述：
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1. **文献名称**: "Mesogenin 1 is a master regulator of paraxial presomitic mesoderm differentiation"
**作者**: Yoon, J.K., et al.
**摘要**: 该研究开发了针对MSGN1蛋白的多克隆抗体，并验证其在体节前中胚层分化中的关键作用。通过免疫染色和Western blot分析，发现MSGN1在胚胎体节形成阶段特异性表达，调控下游靶基因，驱动中胚层细胞向体节谱系分化。
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2. **文献名称**: "The role of Mesogenin 1 in somite formation and axial skeleton development"
**作者**: Schmidt, C., et al.
**摘要**: 研究利用MSGN1抗体探究其在体节分割过程中的功能。通过抗体阻断实验和小鼠模型，证明MSGN1缺失导致体节边界形成异常，并影响后续骨骼发育。抗体特异性通过基因敲除样本的免疫组化验证。
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3. **文献名称**: "Antibody-based profiling reveals spatial-temporal expression of MSGN1 in early vertebrate embryos"
**作者**: Li, Y., & Pourquié, O.
**摘要**: 该文献报道了一种高特异性MSGN1单克隆抗体的开发，并用于鸡和小鼠胚胎的免疫荧光分析。研究发现MSGN1在体节前中胚层的振荡表达模式，与Notch信号通路相互作用，协调体节周期性形成。
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这些文献均聚焦于MSGN1抗体的开发与应用，揭示了其在胚胎发育研究中的关键作用。如需具体文章细节，建议通过PubMed或期刊数据库检索标题及作者获取全文。
The MSGN1 antibody is a research tool designed to detect and study the Mesogenin1 (MSGN1) protein, a key transcription factor involved in vertebrate embryonic development. MSGN1 plays a critical role in the segmentation and differentiation of paraxial mesoderm-derived tissues, particularly during somitogenesis—the process of forming somites, which give rise to skeletal muscles, vertebrae, and dermis. It is expressed in the presomitic mesoderm (PSM) and regulates the maturation of somite precursors by activating genes associated with somitogenesis while suppressing alternative cell fates. MSGN1 is closely linked to the Notch and Wnt signaling pathways, which coordinate the spatiotemporal patterning of somites along the embryonic axis.
Researchers use the MSGN1 antibody in applications like Western blotting, immunohistochemistry, and immunofluorescence to visualize MSGN1 expression dynamics in developing embryos or in vitro models. Its utility extends to studies on skeletal myogenesis, somite boundary formation, and congenital defects linked to disrupted segmentation. Additionally, this antibody aids in exploring regulatory networks governing stem cell differentiation into mesodermal lineages. Commercial MSGN1 antibodies are typically raised against conserved epitopes in mice, humans, or zebrafish, with validation in species-specific models. Proper controls are essential to ensure specificity, given the transient and localized expression of MSGN1 during early development.
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