| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, GRASP55, p59, GORASP2, GOLPH6 |
| Entrez GeneID | 26003 |
| WB Predicted band size | 47.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GORASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-214 amino acids from the Central region of human GORASP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GORASP2抗体的3篇参考文献概览：
1. **文献名称**: *GORASP2 regulates Golgi structure and function in post-mitotic neurons*
**作者**: Smith A, et al.
**摘要**: 研究利用GORASP2抗体揭示其在神经元高尔基体形态维持中的作用，敲除GORASP2导致分泌通路异常，影响突触蛋白运输。
2. **文献名称**: *The role of GORASP2 in cancer cell invasion through ERK signaling modulation*
**作者**: Chen L, et al.
**摘要**: 通过GORASP2抗体的免疫印迹和免疫荧光实验，发现其在乳腺癌细胞中高表达，并通过ERK通路促进肿瘤侵袭转移。
3. **文献名称**: *Antibody-specific validation of GORASP2 in CRISPR/Cas9 knockout models*
**作者**: Park JH, et al.
**摘要**: 该文献报道了一种特异性GORASP2抗体的开发，利用CRISPR敲除细胞验证其无交叉反应性，适用于免疫组化和共定位研究。
4. **文献名称**: *GORASP2 deficiency disrupts collagen secretion in fibroblasts*
**作者**: Rodriguez-Boulan E, et al.
**摘要**: 研究使用GORASP2抗体发现其调控成纤维细胞胶原分泌，缺失导致细胞外基质异常，与组织修复障碍相关。
(注：以上文献信息为模拟生成，实际引用需以真实文献为准。)
The GORASP2 (Golgi Reassembly and Stacking Protein 2) antibody is a tool used to detect and study the GORASP2 protein, a key component of the Golgi apparatus. Also known as GRASP55. GORASP2 is one of two paralogs (with GORASP1/GRASP65) in the GRASP family, which play critical roles in maintaining Golgi structure and facilitating vesicle-mediated protein trafficking. GORASP2 is localized to the medial- and trans-Golgi cisternae, where it acts as a scaffolding protein to organize membrane stacking and support Golgi integrity during interphase. During mitosis, GORASP2 is phosphorylated, leading to Golgi disassembly, and later dephosphorylated to aid Golgi reassembly post-cell division. It also participates in unconventional protein secretion and autophagy pathways.
Antibodies targeting GORASP2 are widely used in research to investigate Golgi dynamics, cell cycle regulation, and cellular stress responses. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping to elucidate GORASP2's expression, localization, and interactions with binding partners (e.g., Golgin-45). Dysregulation of GORASP2 has been linked to diseases such as cancer and neurodegenerative disorders, making its study relevant for understanding pathological mechanisms. Commercial GORASP2 antibodies are typically validated for specificity in human, mouse, or rat samples, aiding cross-species comparative studies.
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