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产品描述：人蛋白精氨酸脱亚氨酶4(PADI4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液样本中的PADI4含量。PADI4 是重要靶点。它能使蛋白质发生瓜氨酸化，在类风湿关节炎等疾病中起关键作用。研究发现其异常表达和活性与疾病的发生发展密切相关，针对 PADI4 开展抑制研究，有望为疾病治疗提供新的策略和药物。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中PADI4相关分子机制研究、药物筛选模型中酶活性调控评估，或疾病状态下生物标志物的动态监测分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 3.5.3.15 ELISA Kit; HL 60 PAD ELISA Kit; HL-60 PAD ELISA Kit; PAD 4 ELISA Kit; PAD ELISA Kit; PADI 4 ELISA Kit; PADI 5 ELISA Kit; PADI H protein ELISA Kit; Padi4 ELISA Kit; PADI4_HUMAN ELISA Kit; PADI5 ELISA Kit; PDI 4 ELISA Kit; PDI 5 ELISA Kit; PDI4 ELISA Kit; PDI5 ELISA Kit; Peptidyl arginine deiminase type IV ELISA Kit; Peptidyl arginine deiminase type V ELISA Kit; Peptidylarginine deiminase IV ELISA Kit; Protein arginine deiminase ELISA Kit; Protein arginine deiminase type 4 ELISA Kit; Protein arginine deiminase type IV ELISA Kit; Protein-arginine deiminase type IV ELISA Kit; Protein-arginine deiminase type-4 ELISA Kit
缩写：PADI4
Uniprot No.：Q9UM07
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7192.6782.6992.60952.2232.1892.2062.1162.51.4271.4021.4151.3251.250.7820.7630.7730.6830.6250.4070.3790.3930.3030.3120.2490.2390.2440.1540.1560.1580.1480.1530.06300.0930.0870.090 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PADI4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled PADI4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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