| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G-rich sequence factor 1 |
| Entrez GeneID | 2926 |
| WB Predicted band size | Calculated MW: 53 kDa; Observed MW: 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human GRSF1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于GRSF1抗体的3篇参考文献，按研究领域和抗体应用方向分类：
1. **文献名称**: *"GRSF1 regulates RNA processing in mitochondrial RNA granules"*
**作者**: Antonicka, H., et al.
**摘要**: 研究揭示了GRSF1在线粒体RNA颗粒中的功能，利用特异性抗体证实GRSF1与线粒体mRNA的结合，并调控其稳定性及翻译效率。
2. **文献名称**: *"The RNA-binding protein GRSF1 modulates influenza virus infection by post-transcriptional control of viral gene expression"*
**作者**: Okamoto, M., et al.
**摘要**: 通过GRSF1抗体进行免疫沉淀和蛋白质定位分析，发现GRSF1通过结合流感病毒RNA调控病毒复制，影响宿主抗病毒反应。
3. **文献名称**: *"GRSF1 suppresses tumor progression by inhibiting aerobic glycolysis in lung adenocarcinoma"*
**作者**: Chen, Y., et al.
**摘要**: 使用GRSF1抗体检测肺癌细胞中蛋白表达水平，发现其通过抑制糖酵解通路抑制肿瘤生长，机制涉及与代谢相关mRNA的相互作用。
4. **文献名称**: *"Dynamic localization of GRSF1 during cellular stress and its interaction with stress granules"*
**作者**: Thomas, M.G., et al.
**摘要**: 利用GRSF1抗体进行免疫荧光分析，证明其在氧化应激条件下转位至应激颗粒，参与细胞应激反应的RNA调控网络。
(注：以上文献信息为模拟示例，实际引用需核对真实数据库及原文。)
The GRSF1 (G-rich RNA sequence-binding factor 1) antibody is a tool used to detect and study the GRSF1 protein, a member of the RNA-binding protein family. GRSF1 is involved in post-transcriptional gene regulation, particularly in mitochondrial RNA processing, mRNA transport, and translation. It contains three quasi-RRM (qRRM) domains that enable binding to G-rich RNA motifs, facilitating its role in RNA metabolism and stability. Research has linked GRSF1 to cellular stress responses, viral replication (e.g., influenza A), and mitochondrial function, making it a focus in studies on cancer, neurodegeneration, and viral pathogenesis.
The GRSF1 antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to analyze protein expression, localization, and interactions. It helps investigate GRSF1's regulatory mechanisms in diseases, such as its overexpression in certain cancers or its role in modulating viral RNA dynamics. Commercial GRSF1 antibodies are typically produced in rabbits or mice using immunogenic peptide sequences specific to conserved regions of the protein. Validation includes testing for specificity via knockout cell lines or siRNA-mediated knockdown. Its application advances understanding of RNA-protein interactions in cellular homeostasis and disease pathways.
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