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产品描述：人Ephrin-B2(EFNB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EFNB2含量。EFNB2 是一个重要的靶点。它在细胞信号传导等生理过程中发挥关键作用。研究发现其信号通路异常与多种疾病发生发展相关，如肿瘤血管生成等。深入研究 EFNB2 及相关机制，有助于揭示疾病发病机理，为开发针对性治疗药物提供理论依据。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞的功能研究、动物模型组织分析及生物标志物探索等科研领域，为探究EFNB2在生理病理过程中的调控机制提供灵敏的定量检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EFNB2; EPLG5; HTKL; LERK5; Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L
缩写：EFNB2
Uniprot No.：P52799
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EFNB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human EFNB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 84-91 | ||||
| EDTA plasma (n=4) | 103 | 100-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1082.1682.1381.9625001.7041.8041.7541.5782501.2811.2621.2721.0961250.7840.7680.7760.60062.50.5620.5310.5470.37131.20.3940.3880.3910.21515.60.2820.2860.2840.10800.1780.1740.176 | ||||||
货期：3-5 working days
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