首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胶原吡啶交联(PYD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的PYD含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于骨代谢机制研究、抗骨质疏松药物筛选、关节炎动物模型评估等科研领域。特别针对尿液样本的兼容性设计，为无创性骨代谢动态监测提供了便捷方案，可满足体外培养体系检测、疾病模型纵向观察等实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PYD
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 88-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 80-94 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of human PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 85-99 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.5012.4502.4762.3434001.9342.0091.9721.8392001.2951.3011.2981.1651000.7180.7750.7470.614500.4830.4950.4890.356250.3310.3510.3410.20812.50.2260.2350.2310.09800.1360.1290.133 | ||||||
货期：3-5 working days
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