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Rabbit Monoclonal AKAP95 Antibody

  • 中文名: AKAP95抗体
  • 别    名: AKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95
货号: IPDX22249
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95
Entrez GeneID10270
WB Predicted band sizeCalculated MW: 76 kDa; Observed MW: 95 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human AKAP 95
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于AKAP95抗体的3篇参考文献(文献信息为模拟示例,实际引用请核实原文):

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1. **文献名称**: *AKAP95 regulates chromatin dynamics and transcriptional activity during cell cycle progression*

**作者**: Li, X., et al.

**摘要**: 本研究利用AKAP95特异性抗体,通过免疫荧光和染色质免疫沉淀(ChIP)技术,揭示了AKAP95通过结合组蛋白H3和RNA聚合酶II调控基因转录的机制,并证明其在G1/S期转换中的关键作用。

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2. **文献名称**: *A-Kinase Anchoring Protein 95 facilitates mRNA splicing via anchoring protein kinase A to nuclear speckles*

**作者**: Han, H., & Zhou, T.

**摘要**: 通过Western blot和免疫共沉淀(Co-IP)实验,作者发现AKAP95抗体能够特异性识别核内剪切体复合物中的AKAP95.并证实其通过招募PKA调控pre-mRNA剪接的分子机制。

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3. **文献名称**: *AKAP95 interacts with viral RNA to promote influenza virus replication*

**作者**: Wang, Y., et al.

**摘要**: 该研究利用AKAP95抗体进行免疫荧光共定位分析,发现AKAP95与流感病毒RNA结合蛋白相互作用,增强病毒RNA的核输出和复制效率,为抗病毒靶点研究提供了新方向。

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**注**:以上文献摘要内容为示例性概括,实际研究需参考真实发表的论文。建议通过PubMed、Google Scholar等平台以关键词“AKAP95 antibody”或“AKAP8”检索最新文献。

背景信息

AKAP95 (A-kinase anchoring protein 95) is a multifunctional scaffolding protein belonging to the AKAP family, which organizes signaling complexes by anchoring protein kinase A (PKA) and other enzymes to specific cellular compartments. Primarily localized in the nucleus, AKAP95 plays critical roles in cell cycle regulation, chromatin remodeling, RNA processing, and transcriptional regulation. It interacts with PKA, histone acetyltransferases, and components of the spliceosome, facilitating cross-talk between signaling pathways and epigenetic modifiers. Studies suggest its involvement in mitotic progression, where it helps condense chromosomes and maintain genomic stability.

AKAP95 antibodies are essential tools for investigating its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study AKAP95's role in cellular processes and disease contexts. Dysregulation of AKAP95 has been linked to cancers, including leukemia and solid tumors, where its overexpression may promote proliferation or metastasis. Researchers also utilize these antibodies to explore AKAP95's non-canonical functions, such as its interaction with non-coding RNAs or involvement in viral infection responses. Commercial AKAP95 antibodies are typically raised against conserved epitopes, often validated for specificity across human and model organisms. However, users should confirm antibody performance due to potential cross-reactivity with related AKAP family members.

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