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Intra-assay Precision (Precision within an assay): CV%<15% | |||||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||
Inter-assay Precision (Precision between assays): CV%<15% | |||||||||
Three samples of known concentration were tested in twenty assays to assess. |
Test parameter | specification | test result | |||||
Positive control | >0.8 | 1.895 | |||||
Negative control | <0.2 | 0.135 | |||||
Positive rate | 10,Positive | 100% | |||||
Negative rate | 10,Negative | 100% |
A 96-well Assay plate --The 96-well plate has been pre-coated with PTH 1-34.
Negative Control (1 x 800 μl) --Eliminate false positive
Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of experimental results.
Sample Diluent (2x 20 ml) --Dilute the sample to an appropriate concentration.
HRP-conjugated Immunoglobulin (1 x 10ml)--Bind to the PTH 1-34 antibody and HRP catalyzes the TMB to elicit a chromogenic reaction.
HRP-conjugate Diluent (2x 20 ml) --(1 x 20ml)
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
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