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产品描述：人甲状旁腺激素(PTH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTH含量。PTH（甲状旁腺激素）是一种由甲状旁腺分泌的多肽激素，主要作用是调节血钙和血磷水平。其研究机制涉及通过激活受体来调节钙磷代谢，对骨骼和肾脏功能至关重要。PTH过多或不足可能导致钙磷代谢紊乱，引发相关疾病。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外实验环境下探究PTH在内分泌调控、骨代谢研究、疾病模型构建等领域的生物学作用，也可用于评估药物或干预措施对PTH表达的影响，为相关基础研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：hPTH ELISA Kit; Parathormone ELISA Kit; Parathyrin ELISA Kit; Parathyroid hormone 1 ELISA Kit; Parathyroid hormone ELISA Kit; Prepro PTH ELISA Kit; Preproparathyroid hormone ELISA Kit; PTH ELISA Kit; PTH1 ELISA Kit; PTH1 receptor ELISA Kit; PTH1R ELISA Kit; PTHR ELISA Kit; PTHR1 ELISA Kit; PTHY_HUMAN ELISA Kit
缩写：PTH
Uniprot No.：P01270
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of human PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 110 | 104-113 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.6742.5982.6362.52320002.1652.1342.1502.03710001.3481.3331.3411.2285000.7390.7540.7470.6342500.3470.3340.3410.2281250.2820.2780.2800.16762.50.1890.1830.1860.07300.1120.1140.113 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PTH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled PTH antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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