首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人基质金属蛋白酶13(MMP-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的MMP13含量。MMP13是一种重要的靶点。它属于基质金属蛋白酶家族，在多种生理过程如组织重塑等中发挥作用。异常表达与关节炎、癌症等疾病的发生发展密切相关。其研究机制集中于调控其活性及表达，以开发治疗相关疾病的药物。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究MMP - 13在炎症反应、组织修复或疾病模型中的动态变化，为探索MMP - 13在骨代谢调控、肿瘤微环境调节等领域的科研项目提供可靠工具，仅限科学研究使用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLG 3 ELISA Kit; CLG3 ELISA Kit; Collagenase 3 ELISA Kit; Collagenase3 ELISA Kit; MANDP1 ELISA Kit; Matrix metallopeptidase 13 (collagenase 3) ELISA Kit; Matrix Metalloproteinase 13 ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP 13 ELISA Kit; MMP-13 ELISA Kit; Mmp13 ELISA Kit; MMP13_HUMAN ELISA Kit
缩写：MMP13
Uniprot No.：P45452
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 84-97 | |||||
回收率：
| The recovery of human MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.6422.8422.7422.63852.1582.3452.2522.1482.51.6021.5891.5961.4921.250.9240.9440.9340.8300.6250.5440.5870.5660.4620.3120.3250.3460.3360.2320.1560.2460.2500.2480.14400.1070.1010.104 | ||||||
货期：3-5 working days
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