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产品描述：人14-3-3 ζ/δ蛋白(YWHAZ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的YWHAZ含量。YWHAZ 即 14-3-3ζ 蛋白，广泛参与细胞信号转导。其背景在于它是 14-3-3 蛋白家族成员，与肿瘤发生、发展关系密切。研究机制上，它可结合多种磷酸化蛋白，调控细胞增殖、凋亡、迁移等过程，有望成为肿瘤治疗新靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，为研究YWHAZ在疾病模型中的功能调控、药物干预效果评估或生物标志物筛选等科研场景提供精准定量工具，实验数据可辅助揭示目标蛋白在特定生理或病理条件下的动态变化规律。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：14 3 3 delta ELISA Kit; 14 3 3 protein zeta/delta ELISA Kit; 14 3 3 protein/cytosolic phospholipase A2 ELISA Kit; 14 3 3 zeta ELISA Kit; 14-3-3 protein zeta/delta ELISA Kit; 1433Z_HUMAN ELISA Kit; Epididymis luminal protein 4 ELISA Kit; Epididymis secretory protein Li 3 ELISA Kit; HEL S 3 ELISA Kit; HEL4 ELISA Kit; KCIP-1 ELISA Kit; KCIP1 ELISA Kit; MGC111427 ELISA Kit; MGC126532 ELISA Kit; MGC138156 ELISA Kit; Phospholipase A2 ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; delta polypeptide ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta polypeptide ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein; zeta polypeptide ELISA Kit; YWHAD ELISA Kit; YWHAZ ELISA Kit
缩写：YWHAZ
Uniprot No.：P63104
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-99 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-100 | |||||
回收率：
| The recovery of human YWHAZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-103 | ||||
| EDTA plasma (n=4) | 92 | 88-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.4542.4882.4712.334 202.0492.0192.0341.897 101.4411.3941.4181.281 50.9080.8680.8880.751 2.50.5950.5570.5760.439 1.250.3520.3560.3540.217 0.6250.2650.2550.2600.123 00.1360.1370.137 | ||||||
货期：3-5 working days
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