首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人14-3-3蛋白eta(YWHAH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的YWHAH含量。YWHAH 是一种重要靶点。它参与细胞内多种信号通路调控，在细胞的生长、存活、凋亡等过程中发挥关键作用。目前围绕它的研究主要集中于剖析其在疾病发生发展中的作用机制，为开发针对该靶点的治疗药物提供理论依据。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于神经生物学、肿瘤微环境研究及疾病分子机制探索等方向，如阿尔茨海默病脑组织样本分析、癌症患者血浆生物标志物检测或体外模型蛋白表达调控实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：14 3 3 protein eta ELISA Kit; 14-3-3 eta ELISA Kit; 14-3-3 protein eta ELISA Kit; 1433F_HUMAN ELISA Kit; Brain protein 14-3-3; eta isoform ELISA Kit; HGNC:12853 ELISA Kit; Protein AS1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; eta isoform ELISA Kit; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; YWHA 1 ELISA Kit; YWHA1 ELISA Kit; Ywhah ELISA Kit
缩写：YWHAH
Uniprot No.：Q04917
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 86-105 | |||||
回收率：
| The recovery of human YWHAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-98 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6322.7562.6942.592202.2242.3762.3002.198101.6461.7481.6971.59550.9340.9500.9420.8402.50.5240.5080.5160.4141.250.3560.3620.3590.2570.6250.2420.2450.2440.14200.1030.1010.102 | ||||||
货期：3-5 working days
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