| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDCA1; CT106; hNuf2; hNuf2R; hsNuf2; NUF2; NUF2R |
| Entrez GeneID | 83540 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Nuf2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Nuf2抗体的模拟参考文献示例(仅供参考，实际文献请通过学术数据库查询)：
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1. **文献名称**：*Nuf2 Antibody Application in Mitotic Chromosome Alignment Studies*
**作者**：Li, R., et al.
**摘要**：研究利用Nuf2特异性抗体，通过免疫荧光技术揭示Nuf2蛋白在有丝分裂中调控染色体排列的分子机制，并证明其与微管结合的关键结构域缺失会导致染色体分离异常。
2. **文献名称**：*Nuf2 as a Prognostic Biomarker in Colorectal Cancer: Validation by Antibody-based Assays*
**作者**：Tanaka, H., et al.
**摘要**：通过免疫组化分析Nuf2在结直肠癌中的表达，发现高表达Nuf2与患者不良预后显著相关，提示其作为潜在治疗靶点的价值，并验证了抗体的特异性与灵敏度。
3. **文献名称**：*Functional Characterization of Nuf2 in Spindle Assembly Checkpoint via RNAi and Antibody Blockade*
**作者**：Zhang, Y., et al.
**摘要**：结合RNA干扰和Nuf2抗体阻断实验，证明Nuf2在纺锤体检查点激活中的双重作用，抗体应用为研究其功能提供了体外实验依据。
4. **文献名称**：*Development of a Novel Monoclonal Nuf2 Antibody for High-Throughput Drug Screening*
**作者**：Smith, J., et al.
**摘要**：报道一种新型Nuf2单克隆抗体的开发，用于高通量筛选靶向Ndc80复合体的抗癌化合物，并验证其在药物筛选平台中的实用性。
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**注意**：以上文献为模拟示例，实际研究需通过PubMed、Web of Science等平台检索。建议结合关键词“Nuf2 antibody”、“kinetochore”或“mitosis”查找最新文献。
**Background of Nuf2 Antibody**
Nuf2 (Nuclear filamentous protein 2) is a critical component of the Ndc80 complex, a conserved kinetochore-associated protein complex essential for proper chromosome segregation during mitosis and meiosis. The Ndc80 complex, comprising Nuf2. Hec1. Spc24. and Spc25. mediates the attachment of spindle microtubules to kinetochores, ensuring accurate chromosome alignment and segregation. Nuf2 specifically interacts with Hec1 to form a heterodimer that binds microtubules, playing a pivotal role in maintaining kinetochore-microtubule stability and signaling the spindle assembly checkpoint.
Dysregulation of Nuf2 is linked to chromosomal instability, aneuploidy, and carcinogenesis. Overexpression of Nuf2 has been observed in various cancers, including hepatocellular carcinoma and breast cancer, and is often correlated with poor prognosis, suggesting its potential as a biomarker or therapeutic target.
Nuf2 antibodies are vital tools for studying mitotic mechanisms, kinetochore dynamics, and cell cycle regulation. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect Nuf2 expression, localization, and interactions in cellular and disease models. These antibodies also contribute to research exploring the relationship between kinetochore dysfunction, genomic instability, and cancer progression. By enabling precise analysis of Nuf2's role in mitosis, these reagents advance our understanding of cell division errors and their implications in human health.
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