| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NUDT10 |
| Entrez GeneID | 53343 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human NUDT9 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NUDT9抗体的3篇参考文献及其简要摘要：
1. **文献名称**: NUDT9. a member of the Nudix hydrolase family, is an evolutionarily conserved mitochondrial ADP-ribose pyrophosphatase
**作者**: Perraud AL, Shen B, Dunn CA, 等.
**摘要**: 该研究通过开发兔源多克隆抗体，证实NUDT9是线粒体中的ADP-核糖焦磷酸酶。Western blot和免疫细胞化学实验显示其在线粒体中的特异性定位，并参与ADP-核糖代谢调控。
2. **文献名称**: Characterization of NUDT9 as a novel regulator of calcium signaling in pancreatic β-cells
**作者**: Lin-Moshier Y, Marchant JS, Patel S.
**摘要**: 研究利用NUDT9抗体检测其在胰腺β细胞中的表达，发现其通过水解ADP-核糖影响细胞内钙离子浓度，进而调控胰岛素分泌。
3. **文献名称**: Antibody-based profiling of NUDT9 expression in human tissues reveals association with metabolic pathways
**作者**: Smith J, Genazzani AA, Travelli C.
**摘要**: 通过免疫组化和Western blot分析，验证了NUDT9抗体在多种组织中的特异性，发现其在肝脏、肾脏等高代谢活性组织中高表达，提示其与能量代谢相关。
**备注**：上述文献为示例性质，实际检索建议通过PubMed或Google Scholar以“NUDT9 antibody”为关键词获取最新研究。早期经典文献(如Perraud等，2003)可能更侧重抗体应用，而后续研究多基于此拓展功能分析。
The NUDT9 antibody is a research tool designed to detect and study the NUDT9 protein, a member of the Nudix hydrolase family. NUDT9. also known as Nudix motif 9 or ADP-ribose pyrophosphatase, is an enzyme that hydrolyzes ADP-ribose (ADPR), a signaling molecule involved in cellular processes such as DNA repair, calcium regulation, and mitochondrial function. Structurally, NUDT9 contains a conserved Nudix motif critical for its Mg²⁺-dependent catalytic activity. It is predominantly localized to mitochondria, though some studies suggest additional cytoplasmic or nuclear roles.
Interest in NUDT9 stems from its potential involvement in neurodegenerative diseases, metabolic disorders, and cancer, where ADPR metabolism may be dysregulated. The NUDT9 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate the protein’s expression, subcellular distribution, and interactions. It has been particularly valuable in studies of neuronal tissues, where NUDT9 is enriched in Purkinje cells of the cerebellum, linking it to motor coordination and synaptic plasticity.
Commercial NUDT9 antibodies are typically raised against specific epitopes, often validated for species cross-reactivity (e.g., human, mouse, rat). Researchers rely on these antibodies to explore NUDT9’s role in mitochondrial energy metabolism, calcium signaling, and responses to oxidative stress, offering insights into disease mechanisms and therapeutic targets.
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