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产品描述：人14-3-3 θ蛋白(YWHAQ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、脑脊液（CSF）样本中的YWHAQ含量。试剂盒检测范围为0.47 ng/mL-30 ng/mL，适用于基础科研中探究YWHAQ在神经系统疾病机制、癌症生物标志物筛选或细胞信号通路调控等领域的表达特征。实验操作兼容多种生物样本类型，尤其为脑脊液等微量样本的检测提供高灵敏度解决方案，可满足体外实验中对蛋白定量分析的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：14-3-3 protein T cell ELISA Kit; 14-3-3 protein T-cell ELISA Kit; 14-3-3 protein tau ELISA Kit; 14-3-3 protein theta ELISA Kit; 1433T_HUMAN ELISA Kit; IC5 ELISA Kit; Protein HS1 ELISA Kit; Protein tau ELISA Kit; YWHAQ tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide ELISA Kit; Ywhaq ELISA Kit
缩写：YWHAQ
Uniprot No.：P27348
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
检测范围：0.47 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human YWHAQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 90-97 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of Human YWHAQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 96-108 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.6132.4192.5162.349152.0182.1142.0661.8997.51.4521.3931.4231.2563.750.8970.8780.8880.7211.880.5410.5180.5300.3630.940.3640.3660.3650.1980.470.2760.2700.2730.10600.1670.1660.167 | ||||||
货期：3-5 working days
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