| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Death domain containing protein p84N5; HPR1; hTREX84; Nuclear matrix protein p84; p84N5; THO complex 1; Tho1; Thoc1 |
| Entrez GeneID | 9984 |
| WB Predicted band size | Calculated MW: 76 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Nuclear Matrix Protein p84 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于THO Complex Subunit 1(THOC1/HPR1)抗体的参考文献示例(注：以下为模拟内容，实际引用需核实原文)：
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1. **"The THO complex mediates mRNA export and prevents transcriptional R-loop accumulation"**
*作者：Strässer, K. et al.*
**摘要**：该研究通过免疫沉淀和Western blot实验，利用THOC1特异性抗体，揭示了THO复合体在mRNA输出中的作用，并证明其缺失会导致R-loop形成异常，影响基因组稳定性。
2. **"THOC1 overexpression correlates with poor prognosis in colorectal cancer"**
*作者：Jimeno, S. et al.*
**摘要**：文章使用抗THOC1抗体进行免疫组化分析，发现THOC1在结直肠癌组织中高表达，且与患者生存率降低相关，提示其作为潜在肿瘤标志物的可能性。
3. **"Subcellular localization of THO complex subunits in mammalian cells"**
*作者：Heath, C.G. & Reed, R.*
**摘要**：通过免疫荧光和共聚焦显微镜技术，结合THOC1抗体，证实THOC1定位于细胞核内，并参与mRNA的核质转运过程。
4. **"Functional interplay between THO complex subunits and RNA helicases"**
*作者：Reed, R. et al.*
**摘要**：研究利用THOC1抗体进行免疫共沉淀实验，发现THO复合体与其他RNA解旋酶(如UAP56)存在相互作用，共同调控mRNA加工与输出机制。
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建议通过PubMed或Web of Science检索具体文献，并核对实验方法部分是否明确提及抗体使用。
**Background of THO Complex Subunit 1 Antibody**
The THO Complex Subunit 1 (THOC1), also known as HPR1. is a critical component of the evolutionarily conserved THO complex, which plays a pivotal role in mRNA processing, export, and genome stability. The THO complex, composed of subunits THOC1-THOC7. interacts with other transcriptional/export machinery (e.g., TREX, ALYREF) to facilitate cotranscriptional mRNA packaging, prevent R-loop formation, and ensure efficient nuclear export of spliced transcripts.
THOC1 antibodies are essential tools for studying these molecular mechanisms. They enable the detection, quantification, and localization of THOC1 in techniques like Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies are often validated for specificity across species (e.g., human, mouse, rat) and are used to explore THOC1's role in transcription-coupled processes, DNA repair, and cellular stress responses.
Research highlights THOC1's involvement in diseases. For instance, THOC1 overexpression is linked to cancer progression (e.g., breast, liver) and poor prognosis, while its deficiency is associated with neurodevelopmental disorders. Antibodies against THOC1 thus aid in uncovering its pathophysiological relevance, therapeutic targeting potential, and interactions within broader gene regulatory networks.
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