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产品描述：人14-3-3 γ蛋白(YWHAG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的YWHAG含量。YWHAG作为一个靶点，它属于14 - 3 - 3蛋白家族成员。参与细胞内多种信号转导途径，能与多种磷酸化的蛋白质相互作用，调节细胞的生长、凋亡和代谢等过程。目前相关研究聚焦其在肿瘤等疾病发生发展机制中的作用，以探索新治疗策略。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于体外研究YWHAG在疾病模型、药物干预实验或分子通路研究中的动态变化，为探索其生物学功能及调控机制提供可靠工具，支持科研人员高效开展蛋白表达水平分析，满足基础研究中对低丰度靶标蛋白的定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：14 3 3 gamma ELISA Kit; 14 3 3 protein gamma ELISA Kit; 14 3 3 protein gamma subtype ELISA Kit; 14 3 3gamma ELISA Kit; 14-3-3 protein gamma ELISA Kit; 1433G_HUMAN ELISA Kit; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide ELISA Kit; KCIP 1 ELISA Kit; KCIP-1 ELISA Kit; KCIP1 ELISA Kit; N-terminally processed ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide ELISA Kit; Ywhag ELISA Kit
缩写：YWHAG
Uniprot No.：P61981
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 83-93 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of human YWHAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 108 | 102-112 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.6232.7452.6842.578 202.1782.2722.2252.119 101.5311.6331.5821.476 50.9380.9170.9280.822 2.50.5220.5040.5130.407 1.250.3370.3420.3400.234 0.6250.2330.2420.2380.132 00.1050.1060.106 | ||||||
货期：3-5 working days
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