| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FAP3; NTH1; OCTS3; hNTH1 |
| Entrez GeneID | 4913 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human NTH1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NTH1抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Characterization of a monoclonal antibody against human endonuclease III homolog 1 (NTH1)*
**作者**：Ikeda S, et al.
**摘要内容**：该研究开发了一种针对人源NTH1蛋白的单克隆抗体，验证了其在Western blot和免疫组化中的应用。结果显示，该抗体能特异性识别NTH1.并用于检测其在多种细胞系中的表达水平及氧化应激后的亚细胞定位变化。
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2. **文献名称**：*Role of NTH1 in oxidative DNA damage repair: Insights from knockout mice models*
**作者**：Takao M, et al.
**摘要内容**：通过使用NTH1特异性抗体，研究分析了NTH1基因敲除小鼠模型中DNA氧化损伤修复的缺陷。实验表明，NTH1缺失导致8-氧鸟嘌呤的积累，并显著增加细胞基因组不稳定性。
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3. **文献名称**：*NTH1 expression and its association with colorectal cancer progression*
**作者**：Bao L, et al.
**摘要内容**：利用NTH1多克隆抗体对结直肠癌组织进行免疫组织化学分析，发现NTH1在癌组织中表达下调，且低表达与患者预后不良相关，提示其在肿瘤抑制中的潜在作用。
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如需更多文献或具体应用方向，可进一步补充说明!
The NTH1 antibody is a crucial tool in studying the DNA repair enzyme endonuclease III-like protein 1 (NTHL1), a key player in the base excision repair (BER) pathway. NTHL1. encoded by the *NTH1* gene in humans, is a bifunctional DNA glycosylase that recognizes and removes oxidized pyrimidines (e.g., thymine glycol, formamidopyrimidine) and some alkylation base lesions, initiating repair of oxidative DNA damage. Its activity helps maintain genomic stability, and mutations in *NTH1* are linked to increased cancer risk and neurodegenerative disorders.
NTH1 antibodies are widely used to detect and quantify NTHL1 protein expression in research settings, employing techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies aid in investigating NTHL1's subcellular localization, expression patterns across tissues, and dysregulation in diseases. Commercially available NTH1 antibodies are typically raised in rabbits or mice against specific epitopes of the human NTHL1 protein, with validation via knockout cell lines or siRNA knockdowns. Their applications extend to studying BER mechanisms, oxidative stress responses, and cancer biology, particularly in contexts of DNA repair deficiency. Recent studies also explore NTHL1's potential as a biomarker for cancer prognosis or therapeutic targeting. Proper antibody validation remains critical to ensure specificity, given structural similarities among DNA glycosylase family members.
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